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Towards a quantitative assessment of inorganic carbon cycling in photosynthetic microorganisms
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SYSNO ASEP 0510250 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Towards a quantitative assessment of inorganic carbon cycling in photosynthetic microorganisms Author(s) Müller, S. (AT)
Zavřel, Tomáš (UEK-B) RID, SAI, ORCID
Červený, Jan (UEK-B) RID, ORCID, SAISource Title Engineering in Life Sciences. - : Wiley - ISSN 1618-0240
Roč. 19, č. 10 (2019), s. 1-13Number of pages 13 s. Language eng - English Country DE - Germany Keywords carbonate chemistry ; computational modeling ; cyanobacteria ; futile cycles ; photosynthesis Subject RIV EF - Botanics OECD category Plant sciences, botany R&D Projects LO1415 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Research Infrastructure CzeCOS II - 90061 - Ústav výzkumu globální změny AV ČR, v. v. i. Method of publishing Open access Institutional support UEK-B - RVO:86652079 UT WOS 000502733800001 DOI 10.1002/elsc.201900061 Annotation Photosynthetic organisms developed various strategies to mitigate high light stress. For instance, aquatic organisms are able to spend excessive energy by exchanging dissolved carbon dioxide (dCO2) and bicarbonate (HCO3-) with the environment. Simultaneous uptake and excretion of the two carbon species is referred to as inorganic carbon cycling (ICC). Often, ICC is indicated by displacements of the extracellular dCO2 signal from the equilibrium value after changing the light conditions. In this work, we additionally use (i) the extracellular pH signal, which requires non- or weakly-buffered medium, and (ii) a dynamic model of carbonate chemistry in the aquatic environment in order to detect and quantitatively describe ICC. Based on simulations and experiments in precisely controlled photobioreactors, we show that the magnitude of the observed dCO2 displacement crucially depends on extracellular pH level and buffer concentration. Moreover, we find that the dCO2 displacement can also be caused by simultaneous uptake of both dCO2 and HCO3- (no ICC). In a next step, the dynamic model of carbonate chemistry allows for a quantitative assessment of cellular dCO2 , HCO3- , and H+ exchange rates from the measured dCO2 and pH signals. Limitations of the method are discussed. Workplace Global Change Research Institute Contact Nikola Šviková, svikova.n@czechglobe.cz, Tel.: 511 192 268 Year of Publishing 2020 Electronic address https://onlinelibrary.wiley.com/doi/full/10.1002/elsc.201900061
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