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MAPK p38alpha Kinase Influences Haematopoiesis in Embryonic Stem Cells
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SYSNO ASEP 0509780 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title MAPK p38alpha Kinase Influences Haematopoiesis in Embryonic Stem Cells Author(s) Štefková, K. (CZ)
Hanackova, M. (CZ)
Kučera, J. (CZ)
Radaszkiewicz, K. A. (CZ)
Ambrůzová, Barbora (BFU-R)
Kubala, Lukáš (BFU-R) RID, ORCID
Pacherník, J. (CZ)Number of authors 7 Article number 5128135 Source Title Stem Cells International - ISSN 1687-966X
Roč. 2019, Č. 2019 (2019)Number of pages 17 s. Publication form Online - E Language eng - English Country US - United States Keywords c/ebp Subject RIV EA - Cell Biology OECD category Cell biology Method of publishing Open access Institutional support BFU-R - RVO:68081707 UT WOS 000472008000001 DOI 10.1155/2019/5128135 Annotation The activation of p38alpha kinase mediates cell response to various extracellular factors including many interleukins and growth factors important for haematopoiesis. The role of p38alpha kinase was previously analysed in particular haematopoietic cells. In this study and for the first time, the role of p38alpha kinase in haematopoiesis was studied using a model of continuous haematopoietic development in pluripotent embryonic stem cells in vitro. The expression of transcripts associated with haematopoiesis and the potential for the formation of specific haematopoietic cell colonies were compared between wild-type and mutant p38alpha gene-depleted cells. The absence of p38alpha kinase led to the inhibition of hemangioblast formation during the first step of haematopoiesis. Later, during differentiation, due to the lack of p38alpha kinase, erythrocyte maturation was impaired. Mutant p38-/- cells also exhibited decreased potential with respect to the expansion of granulocyte colony-forming units. This effect was reversed in the absence of erythropoietin as shown by colony-forming unit assay in media for colony-forming unit granulocytes/macrophages. p38alpha kinase thus plays an important role in the differentiation of common myeloid precursor cells into granulocyte lineages. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2020 Electronic address http://downloads.hindawi.com/journals/sci/2019/5128135.pdf
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