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Immunomodulatory effects of cyanobacterial toxin cylindrospermopsin on innate immune cells
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SYSNO ASEP 0509760 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Immunomodulatory effects of cyanobacterial toxin cylindrospermopsin on innate immune cells Author(s) Moosová, Z. (CZ)
Pekarová, Michaela (BFU-R) RID
Šindlerová, Lenka (BFU-R) RID, ORCID
Vašíček, Ondřej (BFU-R) ORCID, RID
Kubala, Lukáš (BFU-R) RID, ORCID
Bláha, L. (CZ)
Adamovský, O. (CZ)Number of authors 7 Source Title Chemosphere. - : Elsevier - ISSN 0045-6535
Roč. 226, JUL 2019 (2019), s. 439-446Number of pages 8 s. Publication form Print - P Language eng - English Country GB - United Kingdom Keywords nf-kappa-b ; reactive oxygen ; oxidative stress ; activation Subject RIV DJ - Water Pollution ; Quality OECD category Environmental sciences (social aspects to be 5.7) R&D Projects GA16-24949S GA ČR - Czech Science Foundation (CSF) Method of publishing Open access with time embargo (22.03.2021) Institutional support BFU-R - RVO:68081707 UT WOS 000468709500048 DOI 10.1016/j.chemosphere.2019.03.143 Annotation Cylindrospermopsin (CYN), a cyanobacterial toxin, is an important water pollutant with broad biological activity. It has been known mainly from tropical areas, but the area of occurrence of its producers is spreading to temperate climates. It can be found in high concentrations in the environment as well as in purified drinking waters. The aim of the study is to bring a basic information on the ability of CYN to interfere with mammalian innate immunity cells and thus increase the understanding of the immunomodulatory potency of CYN. This study investigated whether immune cells can be a target of CYN either alone or in combination with a model immunomodulatory agent, lipopolysaccharide (LPS). We examined the effects on cellular viability and inflammation signaling of CYN on murine macrophage-like RAW 264.7 cells. Macrophages were treated either with pure toxin (1 mu M) or together with a known stimulator of immunologically active cells, bacterial or cyanobacterial LPS. CYN has had a significant effect on production on pro-inflammatory mediator tumor necrosis factor alpha (TNF-alpha) which correlates with its effect on reactive oxygen species (ROS) production. We found that CYN potentiated the effect of bacterial and cyanobacterial LPS that was documented by activation of inflammatory signaling pathways including mitogen-activated protein kinase p38 as well as consequent expression of inducible nitric oxide synthase (iNOS) and increased production of pro-inflammatory mediators such as nitric oxide (NO), TNF-alpha, interleukin-6 (IL-6). Our study brings one of the first information that contributes to the elucidation of immunomodulatory role of CYN in macrophages under normal and pro-inflammatory conditions. (C) 2019 Elsevier Ltd. All rights reserved. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2020 Electronic address https://www.sciencedirect.com/science/article/pii/S0045653519305892?via%3Dihub
Number of the records: 1