Number of the records: 1

PLANT CELL MORPHOGENESIS: METHODS AND PROTOCOLS, 2ND EDITION

SYSNO ASEP	0509656
Document Type	M - Monograph Chapter
R&D Document Type	Monograph Chapter
Title	Using FM dyes to study endomembranes and their dynamics in plants and cell suspensions
Author(s)	Jelínková, Adriana (UEB-Q)_{RID, ORCID} Malínská, Kateřina (UEB-Q)_{RID, ORCID} Petrášek, Jan (UEB-Q)_{RID, ORCID}
Number of authors	3
Source Title	PLANT CELL MORPHOGENESIS: METHODS AND PROTOCOLS, 2ND EDITION. - TOTOWA : HUMANA PRESS INC., 2019 / Cvrčková F. ; Žárský Viktor - ISSN 1064-3745 - ISBN 978-1-4939-9469-4
Pages	roč. 1992 (2019), s. 173-187
Number of pages	15 s.
Number of copy	2500
Number of pages	380
Publication form	Print - P
Language	eng - English
Country	US - United States
Keywords	Arabidopsis thaliana root tip ; by-2 ; Endocytosis ; Endomembranes ; FM styryl dyes ; Microscopy ; Plasma membrane
Subject RIV	EB - Genetics ; Molecular Biology
OECD category	Cell biology
R&D Projects	LM2015062 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	EF16_013/0001775 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	UEB-Q - RVO:61389030
UT WOS	000487932800012
EID SCOPUS	85066796645
DOI	10.1007/978-1-4939-9469-4_11
Annotation	FM (Fei-Mao) styryl dyes are commonly used for the fluorescence imaging of plasma membrane (PM) and endocytosis in vivo. Thanks to their amphiphilic character, these dyes are incorporated in the outer leaflet of the PM lipid bilayer and emit fluorescence in its hydrophobic environment. The endocytic pathway of FM dye uptake starts with rapid PM staining and continues in PM invaginations and membrane vesicles during endocytosis, followed by staining of trans-Golgi network (TGN) and ending in tonoplast (vacuolar membrane). FM dyes do not stain endoplasmic reticulum and nuclear membrane. The time-lapse fluorescence microscopy could track endocytic vesicles and characterize the rate of endocytosis in vivo. On the other hand, fixable FM dyes (FX) can be used for the visualization of particular steps in the FM dye uptake in situ. Staining with FM dyes and subsequent microscopic observations could be performed on both tissue and cellular level. Here, we describe simple procedures for the effective FM dye staining and destaining in root tip of Arabidopsis thaliana seedlings and suspension-cultured tobacco cells.
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2020

Number of the records: 1