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Isotopic labelling for sensitive detection of nanoparticle uptake and translocation in plants from hydroponic medium and soil
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SYSNO ASEP 0509269 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Isotopic labelling for sensitive detection of nanoparticle uptake and translocation in plants from hydroponic medium and soil Author(s) Nath, J. (IL)
Dror, I. (IL)
Landa, Přemysl (UEB-Q) RID, ORCID
Moťková, Kateřina (UEB-Q) ORCID
Vaněk, Tomáš (UEB-Q) RID, ORCID
Berkowitz, B. (IL)Number of authors 6 Source Title Environmental Chemistry. - : CSIRO Publishing - ISSN 1448-2517
Roč. 16, č. 6 (2019), s. 391-400Number of pages 10 s. Language eng - English Country AU - Australia Keywords copper nanoparticles ; hydroponic cultivation ; isotopically labelled nanoparticles ; silver nanoparticles ; zinc oxide nanoparticles Subject RIV CE - Biochemistry OECD category Environmental biotechnology R&D Projects 8G15003 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UEB-Q - RVO:61389030 UT WOS 000486693000003 EID SCOPUS 85066070588 DOI 10.1071/EN19064 Annotation Unintended releases of nanoparticles (NPs) into agricultural soil have recently raised concerns regarding NP accumulation in plants. In this study, specially synthesised isotopically labelled 107Ag-NPs, 65Cu-NPs and 70ZnO-NPs were exposed to three representative plants (Arabidopsis thaliana, Solanum lycopersicum (tomato) and Phragmites australis (common reed)) in hydroponic cultivation and, separately, to tomato plants cultivated in soil at concentrations of 2 mg L-1. Metal concentrations in all samples were analysed by inductively coupled plasma mass spectrometry following acid digestion. The use of isotopically labelled NPs confirmed that elevated levels of metals were from the NP source used for the experiments. Although the highest concentrations of NPs or metals were detected in roots in both hydroponic and soil cultivations, varied levels of translocation to shoots were observed in different plants under hydroponic cultivation. In soil cultivation, where tomato plants were grown to full maturity, low levels of 107Ag (0.38 mg kg-1) with respect to controls were recorded in tomato fruits, 70Zn showed the highest level of translocation to tomato stems (2.72 mg kg-1) and leaves (13.93 mg kg-1). Furthermore, the amounts of NPs retained in the soil (at different depths) after harvesting tomato plants were also determined, the highest concentrations of respective isotopes (1.25 mg kg-1 of 107Ag, 0.79 mg kg-1 of 65Cu, 4.06 mg kg-1 of 70Zn) were found in the top soil layer (∼3 cm). Analysis of NPs exposed to plants in hydroponic medium indicated that the presence of plants increases the dissolution of NPs. Scanning electron microscopy analysis enabled determination of the location of 107Ag-NPs in the roots of tomato plants grown in soil, these NPs were found to accumulate mainly in the cortical cells. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2020 Electronic address http://dx.doi.org/10.1071/EN19064
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