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A simple and efficient procedure for capillary blood spiking with basic and acidic drugs for subsequent dried blood spot analysis
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SYSNO ASEP 0509090 Document Type A - Abstract R&D Document Type The record was not marked in the RIV R&D Document Type Není vybrán druh dokumentu Title A simple and efficient procedure for capillary blood spiking with basic and acidic drugs for subsequent dried blood spot analysis Author(s) Dvořák, Miloš (UIACH-O)
Ryšavá, Lenka (UIACH-O) RID, ORCID, SAI
Kubáň, Pavel (UIACH-O) RID, ORCIDNumber of authors 3 Source Title CECE 2019. - Gdańsk : University of Gdańsk, 2019
S. 25-25Number of pages 1 s. Publication form Medium - C Action CECE 2019. International Interdisciplinary Meeting on Bioanalysis /16./ Event date 24.09.2019 - 26.09.2019 VEvent location Gdańsk Country PL - Poland Event type WRD Language eng - English Country PL - Poland Keywords dry blood spot ; basic drugs ; DBS technique ; bioanalysis Subject RIV CB - Analytical Chemistry, Separation OECD category Analytical chemistry R&D Projects GA18-13135S GA ČR - Czech Science Foundation (CSF) Institutional support UIACH-O - RVO:68081715 Annotation In the actual contribution, a simple process was developed and optimized for effective IS addition and DBS elution. A group of acidic and/or basic drugs, with different chemical characteristics, was added to the collected capillary blood in different ways and the mixture was transferred onto the DBS collection card (903®Whatman™). Various elution procedures were also examined and are discussed. Direct injection of the eluted DBS sample into CE-UV ensured a rapid and reliable determination of the drugs without the need for any additional pretreatment steps (extraction, preconcentration). The contents of analytes in the dissolved eluates were determined and recoveries >70% were achieved for the optimized elution systems with satisfactory repeatability (RSD <5%). Workplace Institute of Analytical Chemistry Contact Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Year of Publishing 2020
Number of the records: 1