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Pre-treatment and elution of amino acids from dried blood spots for direct determination by capillary electrophoresis
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SYSNO ASEP 0509088 Document Type A - Abstract R&D Document Type The record was not marked in the RIV R&D Document Type Není vybrán druh dokumentu Title Pre-treatment and elution of amino acids from dried blood spots for direct determination by capillary electrophoresis Author(s) Ryšavá, Lenka (UIACH-O) RID, ORCID, SAI
Dvořák, Miloš (UIACH-O)
Miková, B. (CZ)
Kubáň, Pavel (UIACH-O) RID, ORCIDNumber of authors 4 Source Title CECE 2019. - Gdańsk : University of Gdańsk, 2019
S. 20-20Number of pages 1 s. Publication form Medium - C Action CECE 2019. International Interdisciplinary Meeting on Bioanalysis /16./ Event date 24.09.2019 - 26.09.2019 VEvent location Gdańsk Country PL - Poland Event type WRD Language eng - English Country PL - Poland Keywords amino acids ; capillary electrophoresis ; contactless conductivity detection Subject RIV CB - Analytical Chemistry, Separation OECD category Analytical chemistry R&D Projects GA18-13135S GA ČR - Czech Science Foundation (CSF) Institutional support UIACH-O - RVO:68081715 Annotation In this contribution, the process of sampling the DBSs and their subsequent elution with deionized water, methanol, acetonitrile, and optionally with mixtures thereof is shown. Various effects of the co-eluted matrix components on CE-C4D were observed for the selected elution solutions and are comprehensively described. The CE-C4D method was optimized for baseline separation of biogenic amino acids and indicated excellent analytical parameters and high stability of the separation system. CE-C4D was used for the direct analysis of the DBS eluates from healthy donors with amino acid concentrations at physiological levels. Finally, the method was applied to the determination of selected amino acids from DBS samples, which are related to common inborn metabolic disorders, such as maple syrup urine disease and phenylketonuria. Workplace Institute of Analytical Chemistry Contact Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Year of Publishing 2020
Number of the records: 1