Number of the records: 1
Adhesion and growth of adipose tissue-derived stem cells on fibrin assemblies with attached growth factors for tissue engineering of heart valves
- 1.
SYSNO ASEP 0508525 Document Type C - Proceedings Paper (int. conf.) R&D Document Type Conference Paper Title Adhesion and growth of adipose tissue-derived stem cells on fibrin assemblies with attached growth factors for tissue engineering of heart valves Author(s) Filová, Elena (FGU-C) RID, ORCID
Trávníčková, Martina (FGU-C) RID, ORCID, SAI
Knitlová, Jarmila (FGU-C) RID, ORCID
Matějka, Roman (FGU-C) RID, ORCID, SAI
Kučerová, Johanka (UMCH-V)
Riedelová, Zuzana (UMCH-V) ORCID
Brynda, Eduard (UMCH-V) RID
Bačáková, Lucie (FGU-C) RID, ORCIDSource Title NANOCON 2018 - Conference Proceedings, 10th Anniversary International Conference on Nanomaterials - Research and Application. - Ostrava : Tanger Ltd., 2019 - ISBN 978-808729489-5 Pages s. 324-329 Number of pages 6 s. Publication form Print - P Action NANOCON 2018 -International Conference on Nanomaterials - Research and Application /10./ Event date 17.10.2018 - 19.10.2018 VEvent location Brno Country CZ - Czech Republic Event type EUR Language eng - English Country CZ - Czech Republic Keywords adipose tissue-derived stem cells ; fibrin assemblies ; growth factors ; heart valves Subject RIV EI - Biotechnology ; Bionics OECD category Technologies involving the manipulation of cells, tissues, organs or the whole organism (assisted reproduction) Subject RIV - cooperation Institute of Macromolecular Chemistry - Macromolecular Chemistry R&D Projects NV15-29153A GA MZd - Ministry of Health (MZ) GBP108/12/G108 GA ČR - Czech Science Foundation (CSF) Institutional support FGU-C - RVO:67985823 ; UMCH-V - RVO:61389013 UT WOS 000513131900056 EID SCOPUS 85062988207 Annotation Currently used xenogeneic biological heart valve prostheses are decellularized and crosslinked with glutaraldehyde. These grafts usually undergo degeneration and calcification. Pericardium-based heart valve prostheses, re-seeded with autologous cells, i.e. Adipose tissue-derived cells (ASCs) and endothelial cells, could have longer durability and biocompatibility. In order to improve the adhesion of cells and their ingrowth into decellularized pericardium, various fibrin (Fb) layers were developed, i.e. Fb, Fb with covalently bound heparin (H), Fb with either vascular endothelial growth factor (VEGF) or fibroblast growth factor 2 (FGF) in various concentrations (1 ng/ml, 10 ng/ml, 100 ng/ml) or with both VEGF and FGF (100 ng/ml). Growth factors were attached onto Fb via heparin or were adsorbed. ASCs were seeded on theses layers in a DMEM medium supplemented with 2 % of fetal bovine serum, TGFβ1 and BMP-4 (both 2.5 ng/ml), and with ascorbic acid. Cell adhesion and growth/viability was assessed by counted cell number/MTS evaluation. ASCs were stained for differentiation markers of smooth muscle cells, such as alpha-actin, calponin, and myosin heavy chain. On day 7, ASCs on Fb-H-VEGF layers produced both calponin and alpha-actin. An increased FGF concentration caused reduced calponin staining of ASCs. Lack of heparin in fibrin assemblies with growth factors inhibited the production of both alpha-actin and calponin in ASCs. The highest ASCs density/viability was found on Fb-H-VEGF-FGF layer. The proper formulation of fibrin coatings could be favorable for ASCs growth and differentiation and could subsequently support endothelialization of cardiovascular prostheses with endothelial cells. Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2020
Number of the records: 1