Number of the records: 1
Identification of ability to form biofilm in Candida parapsilosis and Staphylococcus epidermidis by Raman spectroscopy
- 1.
SYSNO ASEP 0508215 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Identification of ability to form biofilm in Candida parapsilosis and Staphylococcus epidermidis by Raman spectroscopy Author(s) Rebrošová, K. (CZ)
Šiler, Martin (UPT-D) RID, ORCID, SAI
Samek, Ota (UPT-D) RID, ORCID, SAI
Růžička, F. (CZ)
Bernatová, Silvie (UPT-D) RID, SAI
Ježek, Jan (UPT-D) RID, ORCID, SAI
Zemánek, Pavel (UPT-D) RID, SAI, ORCID
Holá, V. (CZ)Number of authors 8 Source Title Future Microbiology. - : Future Medicine - ISSN 1746-0913
Roč. 14, č. 6 (2019), s. 509-518Number of pages 10 s. Publication form Print - P Language eng - English Country GB - United Kingdom Keywords biofilm ; Candida parapsilosis ; Raman spectroscopy ; rapid diagnostics ; Staphylococcus epidermidis Subject RIV BH - Optics, Masers, Lasers OECD category Optics (including laser optics and quantum optics) R&D Projects GA15-20645S GA ČR - Czech Science Foundation (CSF) LO1212 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) ED0017/01/01 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Limited access Institutional support UPT-D - RVO:68081731 UT WOS 000476570800007 EID SCOPUS 85065886537 DOI 10.2217/fmb-2018-0297 Annotation Aim: Finding rapid, reliable diagnostic methods is a big challenge in clinical microbiology. Raman spectroscopy is an optical method used for multiple applications in scientific fields including microbiology. This work reports its potential in identifying biofilm positive strains of Candida parapsilosis and Staphylococcus epidermidis. Materials & methods: We tested 54 S. epidermidis strains (23 biofilm positive, 31 negative) and 51 C. parapsilosis strains (27 biofilm positive, 24 negative) from colonies on Mueller-Hinton agar plates, using Raman spectroscopy. Results: The accuracy was 98.9% for C. parapsilosis and 96.1% for S. epidermidis. Conclusion: Themethod showed great potential for identifying biofilm positive bacterial and yeast strains. We suggest that Raman spectroscopy might become a useful aid in clinical diagnostics. Workplace Institute of Scientific Instruments Contact Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178 Year of Publishing 2020 Electronic address https://www.futuremedicine.com/doi/10.2217/fmb-2018-0297
Number of the records: 1