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Effects of copper and arsenic stress on the development of Norway spruce somatic embryos and their visualization with the environmental scanning electron microscope
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SYSNO ASEP 0508111 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Effects of copper and arsenic stress on the development of Norway spruce somatic embryos and their visualization with the environmental scanning electron microscope Author(s) Dordevic, B. (CZ)
Neděla, Vilém (UPT-D) RID, ORCID, SAI
Tihlaříková, Eva (UPT-D) RID, ORCID, SAI
Trojan, V. (CZ)
Havel, L. (CZ)Number of authors 5 Source Title New Biotechnology. - : Elsevier - ISSN 1871-6784
Roč. 48, JAN (2019), s. 35-43Number of pages 9 s. Publication form Print - P Language eng - English Country NL - Netherlands Keywords copper ; arsenic ; somatic embryogenesis ; Picea abies (L.) Karst. ; environmental scanning electron microscope Subject RIV JA - Electronics ; Optoelectronics, Electrical Engineering OECD category Plant sciences, botany R&D Projects LO1212 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA14-22777S GA ČR - Czech Science Foundation (CSF) ED0017/01/01 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Limited access Institutional support UPT-D - RVO:68081731 UT WOS 000450366200006 EID SCOPUS 85047354110 DOI 10.1016/j.nbt.2018.05.005 Annotation Somatic embryogenesis is an important biotechnological technique which can be used in studies associated with environmental stress. Four embryogenic cell lines of Norway spruce were grown on media enriched with copper and arsenic in concentration ranges 50-500 mu M and 10-50 mu M, respectively. The effects were observed during subsequent stages of somatic embryogenesis, the characteristics evaluated being proliferation potential, average number of somatic embryos obtained per g/fresh weight, morphology of developed somatic embryos, metal uptake, and microanalysis of macro-and micronutrients uptake. Copper and arsenic at higher concentrations significantly reduced the growth of early somatic embryos. In almost all treatments, the cell line V-1-3 showed the best performance compared with the other lines tested. Environmental scanning electron microscopy was used to visualize and identify morphological abnormalities in the development of somatic embryos. Abnormalities observed were classified into several categories: meristemless somatic embryos, somatic embryos with disrupted meristem, reduced number of cotyledons, single cotyledon and fused cotyledons. With the application of a low temperature method for the environmental scanning electron microscope, samples were stabilized and whole meristems could be investigated in their native state. As far as we are aware, this is the first report of the effect of copper and arsenic during the process of somatic embryogenesis and the first to evaluate the content of macro and micronutrients uptake in Norway spruce. Workplace Institute of Scientific Instruments Contact Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178 Year of Publishing 2020 Electronic address https://www.sciencedirect.com/science/article/pii/S1871678417305174?via%3Dihub
Number of the records: 1