Changes in cryopreserved cell nuclei serve as indicators of processes during freezing and thawing

Kratochvílová, Irena; Kopečná, Olga; Bačíková, Alena; Pagáčová, Eva; Falková, Iva; Follett, S.E.; Elliott, K.W.; Varga, K.; Golan, Martin; Falk, Martin

doi:https://dx.doi.org/10.1021/acs.langmuir.8b02742

Number of the records: 1

Changes in cryopreserved cell nuclei serve as indicators of processes during freezing and thawing

1.

SYSNO ASEP	0507904
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Changes in cryopreserved cell nuclei serve as indicators of processes during freezing and thawing
Author(s)	Kratochvílová, Irena (FZU-D)_{RID, ORCID, SAI} Kopečná, Olga (BFU-R)_ORCID Bačíková, Alena (BFU-R) Pagáčová, Eva (BFU-R)_ORCID Falková, Iva (BFU-R)_ORCID Follett, S.E. (US) Elliott, K.W. (US) Varga, K. (US) Golan, Martin (FZU-D)_RID Falk, Martin (BFU-R)_{RID, ORCID}
Number of authors	10
Source Title	Langmuir. - : American Chemical Society - ISSN 0743-7463 Roč. 35, č. 23 (2019), s. 7496-7508
Number of pages	13 s.
Language	eng - English
Country	US - United States
Keywords	cryopreservation ; chromatin condensation ; cell nuclei membrane
Subject RIV	BO - Biophysics
OECD category	Biophysics
Subject RIV - cooperation	Institute of Biophysics - Physical ; Theoretical Chemistry
R&D Projects	EF16_019/0000760 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	EF16_013/0001406 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	LO1409 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	GA15-05095S GA ČR - Czech Science Foundation (CSF)
	GA16-12454S GA ČR - Czech Science Foundation (CSF)
	NV16-29835A GA MZd - Ministry of Health (MZ)
Method of publishing	Limited access
Institutional support	FZU-D - RVO:68378271 ; BFU-R - RVO:68081707
UT WOS	000471728600021
EID SCOPUS	85056124752
DOI	10.1021/acs.langmuir.8b02742
Annotation	The mechanisms underlying cell protection from cryoinjury are not yet fully understood. Recent biological studies have addressed cryopreserved cell survival but have not correlated the cryoprotection effectiveness with the impact of cryoprotectants on the most important cell structure, the nucleus, and the freeze/thaw process. We identified changes of cell nuclei states caused by different types of cryoprotectants and associate them with alterations of the freeze/thaw process in cells. Namely, we investigated both higher-order chromatin structure and nuclear envelope integrity as possible markers of freezing and thawing processes. Moreover, we analysed in detail the relationship between nuclear envelope integrity, chromatin condensation, freeze/thaw processes in cells and cryopreservation efficiency for DMSO, glycerol, trehalose and antifreeze protein.
Workplace	Institute of Physics
Contact	Kristina Potocká, potocka@fzu.cz, Tel.: 220 318 579
Year of Publishing	2020
Electronic address	https://doi.org/10.1021/acs.langmuir.8b02742

Number of the records: 1