Number of the records: 1
New Method for Automated Disk Diffusion Test
- 1.
SYSNO ASEP 0507186 Document Type C - Proceedings Paper (int. conf.) R&D Document Type Conference Paper Title New Method for Automated Disk Diffusion Test Author(s) Křepelka, P. (CZ)
Kadlec, R. (CZ)
Bartušek, Karel (UPT-D) RID, ORCID, SAI
Jakubec, M. (CZ)Number of authors 4 Source Title PIERS 2014 Guangzhou Proceedings. - Cambridge : The Electromagnetics Academy, 2014 - ISBN 978-1-934142-28-8 Pages s. 192-194 Number of pages 3 s. Publication form Print - P Action Progress in Electromagnetics Research Symposium (PIERS) Event date 25.08.2014 - 28.08.2014 VEvent location Guangzhou Country CN - China Event type WRD Language eng - English Country GB - United Kingdom Keywords disk diffusion test Subject RIV JB - Sensors, Measurment, Regulation OECD category Electrical and electronic engineering Institutional support UPT-D - RVO:68081731 UT WOS 000393225900037 Annotation Microbial resistence to antibiotics is a very important parameter in the selection of a proper therapy and in the control of resistence spreading. One of the most used methods for measuring microbial susceptibility is the disk diffusion test. The test is based on diffusion of concentrated antibiotics from paper disk into agar. Concentration of antibiotics in the agar is dependent on distance from the center of the disc. Near antibiotic disc is concentration much higher due difficult permeation of antibiotics in agar. The diffused antibiotics then inhibit growth of sensitive strains. The zones are usually measured manually or by electronic calipers. Several aproaches for automatic inhibition zone detection have been introduced in recent years. Nevertheless, most of the algorithms are based on a similar radial profile analysis. We have designed a novel image-processing algorithm for measuring the size of inhibition zones of antibiotics based on an analysis of corrected image and rated this image with multicriterial algorithms (based on an observation of the radius profile). The algorithm was tested on 100 clinical isolates, resulting in calculation accuracy of 89% (ratio of success computed radii). If we include alternative radii (to be selected manually), the precision of the calculation rises to 98% (tolerance deviations between manual and automatic measurements were 2 mm). The achieved accuracy was independent of the culture medium (e.g., Muller-Hinton, blood agar, chocolate agar). The main advantage of the algorithm is the invariantness to the tested bacterial strain and culture medium. The new algorithm offers an alternative way to determine inhibition zones and evaluate antimicrobial susceptibility. Workplace Institute of Scientific Instruments Contact Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178 Year of Publishing 2020
Number of the records: 1