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Time evolution of trapped single cell microorganism
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SYSNO ASEP 0507073 Document Type C - Proceedings Paper (int. conf.) R&D Document Type Conference Paper Title Time evolution of trapped single cell microorganism Author(s) Bernatová, Silvie (UPT-D) RID, SAI
Samek, Ota (UPT-D) RID, ORCID, SAI
Obruča, S. (CZ)
Šerý, Mojmír (UPT-D) RID, SAI
Zemánek, Pavel (UPT-D) RID, SAI, ORCID
Márová, I. (CZ)Number of authors 6 Article number 971104 Source Title Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX. Proceedings of SPIE, 9711. - Bellingham : SPIE, 2016 - ISSN 0277-786X - ISBN 978-1-62841-945-0 Number of pages 5 s. Publication form Print - P Action Conference: Conference on Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues /9./ Event date 16.02.2016 - 17.02.2016 VEvent location San Francisco Country US - United States Event type WRD Language eng - English Country US - United States Keywords optical tweezers ; Raman spectroscopy ; 2D correlation analysis ; external stimuli Subject RIV BH - Optics, Masers, Lasers OECD category Microbiology R&D Projects GA15-20645S GA ČR - Czech Science Foundation (CSF) LO1212 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) ED0017/01/01 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UPT-D - RVO:68081731 UT WOS 000378222000001 EID SCOPUS 84978513079 DOI 10.1117/12.2213264 Annotation The combination of optical tweezers and Raman micro-spectroscopy is frequently referred as Raman tweezers. A single focused laser beam is utilized here both as a source of Raman scattering and a source forming an optical trap. Raman tweezers have been recently used in variety of applications in cell biology as a useful tool for non-contact and non-destructive determination of living cells properties. Here we use Raman tweezers to follow response of cells on the length of their cultivation in mineral oil. Analyses of obtained Raman spectra are based on 2D correlation analysis and allow us to determine the chemical background of the cell response in a gentle way. Workplace Institute of Scientific Instruments Contact Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178 Year of Publishing 2020
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