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Resistance of common carp (Cyprinus carpio L.) to oxidative stress after chloramine-T treatment is increased by microalgae carotenoid-rich diet
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SYSNO ASEP 0506897 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Resistance of common carp (Cyprinus carpio L.) to oxidative stress after chloramine-T treatment is increased by microalgae carotenoid-rich diet Author(s) Stará, A. (CZ)
Sergejevova, Magda (MBU-M)
Kozák, P. (CZ)
Masojídek, Jiří (MBU-M) RID, ORCID
Velíšek, J. (CZ)
Kouba, A. (CZ)Source Title Neuroendocrinology Letters - ISSN 0172-780X
Roč. 35, č. 2 (2014), s. 71-80Number of pages 10 s. Language eng - English Country SE - Sweden Keywords fish ; chlorella ; oxidative stress Subject RIV EE - Microbiology, Virology OECD category Microbiology R&D Projects ED2.1.00/03.0110 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support MBU-M - RVO:61388971 UT WOS 000351064900009 Annotation In fish aquaculture, disinfectants are used against bacterial and protozoal infections. These compounds cause oxidative stress that may stimulate the generation of reactive oxygen species, and subsequently the alteration in antioxidant systems of exposed organisms. Antioxidants present in microalgae increase carp resistance to oxidative stress after chemical treatment.
The aim of these experiments was to prove increased resistance of common carp (Cyprinus carpio L.) juveniles fed on experimental diets with microalgae biomass supplement (Algadiets) to oxidative stress caused by a disinfectant chloramine-T. Fish were fed on laboratory-prepared diets containing supplement of Chlorella spp., biomass which containing antioxidants. The year-old fish were acclimatized and fed on basal diet (control group) and the on diets containing 1, 2, 5 and 10% (w/w) of spray-dried Chlorella biomass (Algadiet 1, 2, 5 and 10) for 14 days followed by 6 weeks. Consequently, fish were treated daily with chloramine-T (Chl-T) at concentration of 10 mg.l(-1) for 1 h in three consecutive days. After this treatment, the indices of oxidative stress and antioxidant enzyme activity were assayed in fish gill, muscle and hepatopancreas.
The fish fed on different Algadiets had increased antioxidant enzyme activities of glutathione peroxidase, glutathione reductase and catalase in flesh after the exposure to Chl-T. Higher activities of superoxide dismutase, glutathione peroxidase and glutathione reductase were also observed in the hepatopancreas in all tested concentrations compared to the control group fed on the basal diet. The increased production and activity of antioxidant enzymes confirmed improved protection ability of fish tissues against oxidative damage when microalgae biomass was supplemented to the fish diet which was more pronounced by higher microalgae supplement in Algadiet 5 and 10 where the content of carotenoids was 105 mg and 214 mg per kilogram of fed, respectively.
Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2020 Electronic address http://www.nel.edu/userfiles/articlesnew/1520712135_35_s2_stara_71-80-pdf.pdf
Number of the records: 1