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Electrochemical Detection of p53 Protein Interactions with Plasmid DNAs Modified with Cisplatin Using Immunoprecipitation at Magnetic Microbeads
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SYSNO ASEP 0506734 Document Type C - Proceedings Paper (int. conf.) R&D Document Type Conference Paper Title Electrochemical Detection of p53 Protein Interactions with Plasmid DNAs Modified with Cisplatin Using Immunoprecipitation at Magnetic Microbeads Author(s) Pivoňková, Hana (BFU-R) ORCID
Tichý, Vlastimil (BFU-R) RID
Orság, Petr (BFU-R) ORCID
Šebest, Peter (BFU-R) ORCID
Fojta, Miroslav (BFU-R) RID, ORCIDNumber of authors 5 Source Title XXXIV. Moderní elektrochemické metody sborník přednášek. - Ústí nad Labem : Best servis, 2014 / Navrátil T. ; Fojta M. ; Pecková K. - ISBN 978-80-905221-2-1 Pages č. 2014 (2014), s. 126-130 Number of pages 5 s. Publication form Print - P Action Moderní Elektrochemické Metody /34./ Event date 19.05.2014-23.05.2014 VEvent location Jetřichovice Country CZ - Czech Republic Event type EUR Language eng - English Country CZ - Czech Republic Keywords recognition ; platinum ; binding Subject RIV CG - Electrochemistry OECD category Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis) Institutional support BFU-R - RVO:68081707 UT WOS 000353446900027 Annotation Antineoplastic drug [cis-diamminedichloroplatinum(II)] (cisplatin) forms covalent adducts with DNA. Cisplatin-modified DNA can be determined sensitively using square-wave voltammetry at mercury electrodes. Tumor suppressor protein p53 binds to DNA in different modes, including sequence-and structure-specific ones and these interactions are influenced by modification of the DNA with cisplatin. In this contribution we present a simple immunoprecipitation technique with magnetic beads, followed by voltammetric determination of recovered cisplatinated DNA, for the evaluation of p53 protein binding to DNAs containing various target sites differing in their proneness to being internally modified with the platinum complex. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2020
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