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Electrochemical Analysis of Chemically Modified DNA at Gold Electrodes
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SYSNO ASEP 0506690 Document Type C - Proceedings Paper (int. conf.) R&D Document Type Conference Paper Title Electrochemical Analysis of Chemically Modified DNA at Gold Electrodes Author(s) Vidláková, Pavlína (BFU-R)
Havran, Luděk (BFU-R) RID, ORCID
Fojta, Miroslav (BFU-R) RID, ORCIDNumber of authors 3 Source Title XXXVI. Moderní elektrochemické metody. Sborník přednášek. - Ústí nad Labem : Best servis, 2016 / Navrátil T. ; Fojta M. ; Schwarzová K. - ISBN 978-80-905221-4-5 Pages č. 2016 (2016), s. 282-284 Number of pages 3 s. Publication form Print - P Action Moderní elektrochemické metody /36./ Event date 23.05.2016 - 27.05.2016 VEvent location Jetřichovice Country CZ - Czech Republic Event type WRD Language eng - English Country CZ - Czech Republic Keywords enzymatic incorporation ; nucleic-acids ; immobilization Subject RIV CF - Physical ; Theoretical Chemistry OECD category Physical chemistry R&D Projects GA15-08434S GA ČR - Czech Science Foundation (CSF) GBP206/12/G151 GA ČR - Czech Science Foundation (CSF) Institutional support BFU-R - RVO:68081707 UT WOS 000389898200064 Annotation DNA labeling is used to increase sensitivity of its electrochemical detection. Covalently bound markers based on a complex of osmium tetroxide with 22-bipyridine(Os,bipy) is one of those frequently applied. Os bipy reacts with pyrimidines (mainly thymines) in single stranded regions of DNA, therefore it represents a useful marker for the analysis of DNA structure or labeling of hybridization probes . Another possibility of DNA labeling is the incorporation of chemically modified dNTPs into DNA molecules with using primer extension. DNA labeling in connection with voltammetry at gold electrodes has application potential in development of sensors for studying of DNA structure and interaction. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2020
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