Regulation of Microtubule Nucleation in Mouse Bone Marrow-Derived Mast Cells by Protein Tyrosine Phosphatase SHP-1

Klebanovych, Anastasiya; Sládková, Vladimíra; Sulimenko, Tetyana; Vosecká, Věra; Rubíková, Zuzana; Čapek, Martin; Dráberová, Eduarda; Dráber, Pavel; Sulimenko, Vadym

doi:https://dx.doi.org/10.3390/cells8040345

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Regulation of Microtubule Nucleation in Mouse Bone Marrow-Derived Mast Cells by Protein Tyrosine Phosphatase SHP-1

1.

SYSNO ASEP	0506029
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Regulation of Microtubule Nucleation in Mouse Bone Marrow-Derived Mast Cells by Protein Tyrosine Phosphatase SHP-1
Author(s)	Klebanovych, Anastasiya (UMG-J) Sládková, Vladimíra (UMG-J) Sulimenko, Tetyana (UMG-J) Vosecká, Věra (UMG-J) Rubíková, Zuzana (UMG-J) Čapek, Martin (UMG-J) Dráberová, Eduarda (UMG-J)_{RID, ORCID} Dráber, Pavel (UMG-J)_{RID, ORCID} Sulimenko, Vadym (UMG-J)_{RID, ORCID}
Number of authors	9
Article number	345
Source Title	Cells. - : MDPI Roč. 8, č. 4 (2019)
Number of pages	23 s.
Publication form	Print - P
Language	eng - English
Country	CH - Switzerland
Keywords	gamma-tubulin complexes ; kinase ; phosphorylation ; syk ; fyn ; degranulation ; centrosome ; receptor ; cycle ; lyn
Subject RIV	EB - Genetics ; Molecular Biology
OECD category	Cell biology
R&D Projects	GA16-25159S GA ČR - Czech Science Foundation (CSF)
	GA16-23702S GA ČR - Czech Science Foundation (CSF)
	GA17-11898S GA ČR - Czech Science Foundation (CSF)
	GA18-27197S GA ČR - Czech Science Foundation (CSF)
	LM2015062 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	LO1419 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	LTAUSA17052 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	EF16_013/0001775 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Method of publishing	Open access
Institutional support	UMG-J - RVO:68378050
UT WOS	000467304300059
DOI	10.3390/cells8040345
Annotation	The antigen-mediated activation of mast cells initiates signaling events leading to their degranulation, to the release of inflammatory mediators, and to the synthesis of cytokines and chemokines. Although rapid and transient microtubule reorganization during activation has been described, the molecular mechanisms that control their rearrangement are largely unknown. Microtubule nucleation is mediated bytubulin complexes. In this study, we report on the regulation of microtubule nucleation in bone marrow-derived mast cells (BMMCs) by Src homology 2 (SH2) domain-containing protein tyrosine phosphatase 1 (SHP-1, Ptpn6). Reciprocal immunoprecipitation experiments and pull-down assays revealed that SHP-1 is present in complexes containingtubulin complex proteins and protein tyrosine kinase Syk. Microtubule regrowth experiments in cells with deleted SHP-1 showed a stimulation of microtubule nucleation, and phenotypic rescue experiments confirmed that SHP-1 represents a negative regulator of microtubule nucleation in BMMCs. Moreover, the inhibition of the SHP-1 activity by inhibitors TPI-1 and NSC87877 also augmented microtubule nucleation. The regulation was due to changes intubulin accumulation. Further experiments with antigen-activated cells showed that the deletion of SHP-1 stimulated the generation of microtubule protrusions, the activity of Syk kinase, and degranulation. Our data suggest a novel mechanism for the suppression of microtubule formation in the later stages of mast cell activation.
Workplace	Institute of Molecular Genetics
Contact	Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217
Year of Publishing	2020
Electronic address	https://www.mdpi.com/2073-4409/8/4/345

Number of the records: 1