Number of the records: 1

Oxidized glycerophosphatidylcholines in diabetes through non-targeted metabolomics: Their annotation and biological meaning

SYSNO ASEP	0505648
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Oxidized glycerophosphatidylcholines in diabetes through non-targeted metabolomics: Their annotation and biological meaning
Author(s)	Godzien, J. (ES) Kalaska, B. (PL) Adamska-Patruno, E. (PL) Široká, Jitka (UEB-Q)_ORCID Ciborowski, M. (PL) Kretowski, A. (PL) Barbas, C. (ES)
Number of authors	7
Source Title	Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences. - : Elsevier - ISSN 1570-0232 Roč. 1120, JUL 1 (2019), s. 62-70
Number of pages	9 s.
Language	eng - English
Country	NL - Netherlands
Keywords	Long chain oxidized glycerophosphatidylcholines ; Metabolomics ; oxGPCs ; Oxidation ; t2dm
Subject RIV	CE - Biochemistry
OECD category	Biochemistry and molecular biology
R&D Projects	EF16_019/0000827 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Method of publishing	Open access
Institutional support	UEB-Q - RVO:61389030
UT WOS	000470042300008
EID SCOPUS	85065030133
DOI	10.1016/j.jchromb.2019.04.053
Annotation	Lipid oxidation is one of the most important processes occurring in living cells and has been investigated through stable end-products. Currently, new insights into many physiological and pathophysiological processes provide a measurement of the first products of oxidation, e.g., oxidized glycerophosphatidylcholines (oxGPCs). Here, we evaluate the capacity of untargeted global metabolomics to measure oxGPCs in serum samples. This evaluation covered analytical reproducibility and data quality as well as the ability to capture metabolic alterations in diverse conditions. The analytical evaluation was performed based on the quality control samples, while the comparative analysis was based on the model of the development of type 2 diabetes mellitus (T2DM). The novelty of this approach arises not only from the measurement of oxGPCs instead of lipid peroxide-derived aldehydes but also from the stratification of the patients according to body mass index (BMI). Such a scenario was dictated by the fact that, despite the well-known relationship between obesity and T2DM development, there are lean individuals suffering from T2DM as well as obese people with normal glucose homeostasis. Our results provided evidence to support the ability of nontargeted metabolomics to measure oxGPCs. Comparative analysis of measured oxGPCs revealed differences in the level of oxGPCs either between different stages of disease development (insulin resistance, prediabetes) or BMI groups (normal weight, overweight, obese). The obtained results provided new insights into the metabolic processes leading to the development of T2DM and opened new paths in the investigation of the impact of body mass in T2DM progress.
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2020
Electronic address	http://doi.org/10.1016/j.jchromb.2019.04.053

Number of the records: 1