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High diversity of thermophilic cyanobacteria in Rupite hot spring identified by microscopy, cultivation, single-cell PCR and amplicon sequencing

SYSNO ASEP	0505474
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	High diversity of thermophilic cyanobacteria in Rupite hot spring identified by microscopy, cultivation, single-cell PCR and amplicon sequencing
Author(s)	Strunecký, O. (CZ) Kopejtka, Karel (MBU-M)_{ORCID, RID} Goecke, Franz (MBU-M) Tomasch, J. (DE) Lukavský, Jaromír (BU-J)_{RID, ORCID} Neori, A. (IL) Kahl, S. (DE) Pieper, D. H. (DE) Pilarski, P. (BG) Kaftan, David (MBU-M)_ORCID Koblížek, Michal (MBU-M)_{RID, ORCID}
Source Title	Extremophiles. - : Springer - ISSN 1431-0651 Roč. 23, č. 1 (2019), s. 35-48
Number of pages	14 s.
Language	eng - English
Country	JP - Japan
Keywords	Bulgaria ; Cyanobacteria ; Extremophile
Subject RIV	CE - Biochemistry
OECD category	Biochemistry and molecular biology
Subject RIV - cooperation	Institute of Botany Institute of Botany - Botanics
R&D Projects	GA15-00703S GA ČR - Czech Science Foundation (CSF)
	GA15-00113S GA ČR - Czech Science Foundation (CSF)
	LO1416 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Method of publishing	Limited access
Institutional support	MBU-M - RVO:61388971 ; BU-J - RVO:67985939
UT WOS	000456024600004
EID SCOPUS	85054601300
DOI	10.1007/s00792-018-1058-z
Annotation	Genotypic and morphological diversity of cyanobacteria in the Rupite hot spring (Bulgaria) was investigated by means of optical microscopy, cultivation, single-cell PCR, and 16S rRNA gene amplicon sequencing. Altogether, 34 sites were investigated along the 71-39 degrees C temperature gradient. Analysis of samples from eight representative sites shown that Illumina, optical microscopy, and Roche 454 identified 72, 45 and 19% respective occurrences of all cumulatively present taxa. Optical microscopy failed to detect species of minor occurrence, whereas, amplicon sequencing technologies suffered from failed primer annealing and the presence of species with extensive extracellular polysaccharides production. Amplicon sequencing of the 16S rRNA gene V5-V6 region performed by Illumina identified the cyanobacteria most reliably to the generic level. Nevertheless, only the combined use of optical microscopy, cultivation and sequencing methods allowed for reliable estimate of the cyanobacterial diversity. Here, we show that Rupite hot-spring system hosts one of the richest cyanobacterial flora reported from a single site above 50 degrees C. Chlorogloeopsis sp. was the most abundant at the highest temperature (68 degrees C), followed by Leptolyngbya boryana, Thermoleptolyngbya albertanoae, Synechococcus bigranulatus, Oculatella sp., and Desertifilum sp. thriving above 60 degrees C, while Leptolyngbya geysericola, Geitlerinema splendidum, and Cyanobacterium aponinum were found above 50 degrees C.
Workplace	Institute of Microbiology
Contact	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Year of Publishing	2020
Electronic address	https://link.springer.com/article/10.1007%2Fs00792-018-1058-z

Number of the records: 1