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Extracellular biopolymers produced by Dictyosphaerium family - Chemical and immunomodulative properties
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SYSNO ASEP 0505098 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Extracellular biopolymers produced by Dictyosphaerium family - Chemical and immunomodulative properties Author(s) Halaj, M. (SK)
Paulovičová, E. (SK)
Paulovičová, L. (SK)
Jantová, S. (SK)
Cepák, Vladislav (BU-J) RID
Lukavský, Jaromír (BU-J) RID, ORCID
Capek, P. (SK)Number of authors 7 Source Title International Journal of Biological Macromolecules. - : Elsevier - ISSN 0141-8130
Roč. 121, JAN 2019 (2019), s. 1254-1263Number of pages 10 s. Language eng - English Country GB - United Kingdom Keywords Green microalgae ; Dictyosphaerium ; Extracellular biopolymers ; Immunomodulative effectivity Subject RIV CE - Biochemistry OECD category Biochemistry and molecular biology R&D Projects TE01020080 GA TA ČR - Technology Agency of the Czech Republic (TA ČR) Method of publishing Limited access Institutional support BU-J - RVO:67985939 UT WOS 000452346100140 EID SCOPUS 85055340432 DOI 10.1016/j.ijbiomac.2018.10.116 Annotation Many microalgal species produce a wide range of highly-value products which are interesting for biotechnological applications. Cultivation of microalgal species Dictyosphaerium pulchellum and Dictyosphaerium tetrachotomum, strains Růžicka and Fott resulted yields of 0.2, 0.7 and 1.8 g/L of extracellular biopolymers (EPSs), respectively. All biopolymers were shown to be anionic proteoglycans. The sugar composition analyses of all EPSs showed high contents of hexoses and the presence of partially methylated monosaccharide residues, i.e. hexoses, and deoxy hexoses. The dominant sugar component of all EPSs was found to be galactose. Extracellular microalgal biopolymers were subjected to immunobiological and immunotoxicological evaluation using murine melanoma cancer cells B16, murine fibroblast cell line NIH-3T3, murine macrophages cell line RAW 264.7 and skin construct EpiDerm™ (EPI-200). The EPSs exerted the antiproliferative effectivity treatment of EPS induced proinflammatory cytokines TNF-α, IL-6, IL-12, IL-1β and IL-17, also engaged in anti-cancer immunity. Immunotoxicological studies revealed their non-toxic character and safe application on EpiDerm™. Workplace Institute of Botany Contact Martina Bartošová, martina.bartosova@ibot.cas.cz, ibot@ibot.cas.cz, Tel.: 271 015 242 ; Marie Jakšová, marie.jaksova@ibot.cas.cz, Tel.: 384 721 156-8 Year of Publishing 2020 Electronic address http://hdl.handle.net/11104/0296634
Number of the records: 1