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Unbiased estimation of Norway spruce (Picea abies L. Karst.) chloroplast structure: Heterogeneity within needle mesophyll under different irradiance and [CO 2 ]

SYSNO ASEP	0504785
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Unbiased estimation of Norway spruce (Picea abies L. Karst.) chloroplast structure: Heterogeneity within needle mesophyll under different irradiance and [CO 2 ]
Author(s)	Kubínová, Z. (CZ) Glanc, N. (CZ) Radochová, Barbora (FGU-C)_{RID, ORCID} Lhotáková, Z. (CZ) Janáček, Jiří (FGU-C)_{RID, ORCID} Kubínová, Lucie (FGU-C)_{RID, ORCID} Albrechtová, J. (CZ)
Source Title	Image Analysis and Stereology. - : Društvo za stereologijo in kvantitativno analizo slike - ISSN 1580-3139 Roč. 38, č. 1 (2019), s. 83-94
Number of pages	10 s.
Language	eng - English
Country	SI - Slovenia
Keywords	chloroplast number ; chloroplast ultrastructure ; elevated CO2 ; needle anatomy ; stereology
Subject RIV	GK - Forestry
OECD category	Forestry
R&D Projects	GAP501/10/0340 GA ČR - Czech Science Foundation (CSF)
	LM2015062 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	EF16_013/0001775 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Research Infrastructure	Czech-BioImaging - 90062 - Ústav molekulární genetiky AV ČR, v. v. i. CESNET II - 90042 - CESNET - zájmové sdružení právnických osob
Method of publishing	Open access
Institutional support	FGU-C - RVO:67985823
UT WOS	000464195100009
EID SCOPUS	85065480947
DOI	10.5566/ias.2005
Annotation	The main objective of this study was to find out whether the selected chloroplast characteristics measured in the mesophyll layer nearest to the needle surface (i.e., the first mesophyll layer) could be representative for the whole needle cross section. Two chloroplast sampling approaches were applied on Norway spruce needles during the investigation of the effects of different levels of air CO2 concentration and irradiance: (i) sampling only from the first mesophyll layer, and (ii) systematic uniform random (SUR) sampling. The selected characteristics were: (i) chloroplast area, (ii) starch grain area, and (iii) starch areal density on median chloroplast cross sections, and (iv) chloroplast number per unit of needle volume. It was shown that the first mesophyll layer was not representative for estimating all evaluated characteristics except the chloroplast area. Sampling only there caused obtaining slightly biased results, while SUR sampling gave unbiased estimations at the cost of longer measuring time. The major effect of studied factors was in starch areal density and starch grain area, which were larger in sun needles in elevated CO2 concentration in comparison with sun needles in ambient CO2 concentration. In conclusion, it was demonstrated that the first layer of mesophyll is not always representative for the needle cross section. If technically feasible, SUR is recommended for analysis of chloroplast ultrastructure. The simplified sampling design can be applied, e.g., for comparisons of many different treatments. However, it should be combined with other approaches to characterize the chloroplast function and the results carefully considered and interpreted.
Workplace	Institute of Physiology
Contact	Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
Year of Publishing	2020
Electronic address	https://www.ias-iss.org/ojs/IAS/article/view/2005

Number of the records: 1