Number of the records: 1
Nanocrystalline diamond-based impedance sensors for real-time monitoring of adipose tissue-derived stem cells
- 1.
SYSNO ASEP 0504656 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Nanocrystalline diamond-based impedance sensors for real-time monitoring of adipose tissue-derived stem cells Author(s) Procházka, Václav (FZU-D) ORCID
Matějka, Roman (FGU-C) RID, ORCID, SAI
Ižák, Tibor (FZU-D) RID
Szabó, Ondrej (FZU-D) ORCID, RID
Štěpanovská, Jana (FZU-D)
Filová, Elena (FGU-C) RID, ORCID
Bačáková, Lucie (FGU-C) RID, ORCID
Jirásek, Vít (FZU-D) RID
Kromka, Alexander (FZU-D) RID, ORCID, SAINumber of authors 9 Source Title Colloids and Surfaces B-Biointerfaces. - : Elsevier - ISSN 0927-7765
Roč. 177, May (2019), s. 130-136Number of pages 7 s. Language eng - English Country NL - Netherlands Keywords diamond film ; biosensor ; stem cells ; impedance spectroscopy Subject RIV JB - Sensors, Measurment, Regulation OECD category Electrical and electronic engineering Subject RIV - cooperation Institute of Physiology - Biotechnology ; Bionics R&D Projects NV15-33018A GA MZd - Ministry of Health (MZ) EF16_019/0000760 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Limited access Institutional support FZU-D - RVO:68378271 ; FGU-C - RVO:67985823 UT WOS 000468720700015 EID SCOPUS 85060851825 DOI 10.1016/j.colsurfb.2019.01.048 Annotation Here we present a diamond-based impedance sensor with built-in gold IDT as a promising platform for simultaneous electrical and optical monitoring of adipose tissue-derived stem cells (ASCs). The impedance spectra were collected in a wide frequency from 100 Hz to 50 kHz for 90 h of cell cultivation. Absolute impedance spectra were analyzed in terms of cell properties monitored by a high-resolution digital camera. The sensors detected three phases of cell growth: the phase of cell attachment and spreading, the phase of cell proliferation, and the stationary phase without significant changes in cell number. These results were confirmed by simultaneous live cell imaging. The design of the sensing electrode is discussed, pointing out its enhanced sensitivity for a certain case. The diamond-based sensor appeared to be more sensitive for detecting the cell-substrate interaction in the first phase of cell growth, while the control system was more sensitive in the second phase of cell growth. Workplace Institute of Physics Contact Kristina Potocká, potocka@fzu.cz, Tel.: 220 318 579 Year of Publishing 2020 Electronic address https://doi.org/10.1016/j.colsurfb.2019.01.048
Number of the records: 1