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Novel contribution to clubfoot pathogenesis: The possible role of extracellular matrix proteins

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    SYSNO ASEP0504465
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JČlánek ve WOS
    TitleNovel contribution to clubfoot pathogenesis: The possible role of extracellular matrix proteins
    Author(s) Eckhardt, Adam (FGU-C) RID, ORCID
    Novotný, T. (CZ)
    Doubková, Martina (FGU-C) ORCID, RID
    Hronková, Lucia (FGU-C)
    Vajner, L. (CZ)
    Pataridis, Statis (FGU-C)
    Hadraba, Daniel (FGU-C) RID, ORCID, SAI
    Kulhavá, Lucie (FGU-C) ORCID
    Plencner, Martin (FGU-C) RID, ORCID
    Knitlová, Jarmila (FGU-C) RID, ORCID
    Lišková, Jana (FGU-C) RID, ORCID
    Uhlík, J. (CZ)
    Žaloudíková, M. (CZ)
    Vondrášek, David (FGU-C) ORCID, SAI
    Mikšík, Ivan (FGU-C) RID, ORCID, SAI
    Ošťádal, M. (CZ)
    Source TitleJournal of Orthopaedic Research - ISSN 0736-0266
    Roč. 37, č. 3 (2019), s. 769-778
    Number of pages10 s.
    Languageeng - English
    CountryGB - United Kingdom
    Keywordsclubfoot ; fibrosis, extracellular matrix ; proteomics ; collagen
    Subject RIVFI - Traumatology, Orthopedics
    OECD categoryOrthopaedics
    R&D ProjectsNV17-31564A GA MZd - Ministry of Health (MZ)
    Method of publishingLimited access
    Institutional supportFGU-C - RVO:67985823
    UT WOS000464419100026
    EID SCOPUS85061451671
    DOI10.1002/jor.24211
    AnnotationIdiopathic pes equinovarus (clubfoot) is a congenital deformity of the feet and lower legs. Clubfoot belongs to a group of fibro‐proliferative disorders but its origin remains unknown. Our study aimed to achieve the first complex proteomic comparison of clubfoot contracted tissue of the foot (medial side, n = 16), with non‐contracted tissue (lateral side, n = 13). We used label‐free mass spectrometry quantification and immunohistochemistry. Seven proteins were observed to be significantly upregulated in the medial side (asporin, collagen type III, V, and VI, versican, tenascin‐C, and transforming growth factor beta induced protein) and four in the lateral side (collagen types XII and XIV, fibromodulin, and cartilage intermediate layer protein 2) of the clubfoot. Comparison of control samples from cadavers brought only two different protein concentrations (collagen types I and VI). We also revealed pathological calcification and intracellular positivity of transforming growth factor beta only in the contracted tissue of clubfoot. Most of the 11 differently expressed proteins are strongly related to the extracellular matrix architecture and we assume that they may play specific roles in the pathogenesis of this deformity. These proteins seem to be promising targets for future investigations and treatment of this disease.
    WorkplaceInstitute of Physiology
    ContactLucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
    Year of Publishing2020
    Electronic addresshttps://doi.org/10.1002/jor.24211
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