Number of the records: 1
Novel contribution to clubfoot pathogenesis: The possible role of extracellular matrix proteins
- 1.
SYSNO ASEP 0504465 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Novel contribution to clubfoot pathogenesis: The possible role of extracellular matrix proteins Author(s) Eckhardt, Adam (FGU-C) RID, ORCID
Novotný, T. (CZ)
Doubková, Martina (FGU-C) ORCID, RID
Hronková, Lucia (FGU-C)
Vajner, L. (CZ)
Pataridis, Statis (FGU-C)
Hadraba, Daniel (FGU-C) RID, ORCID, SAI
Kulhavá, Lucie (FGU-C) ORCID
Plencner, Martin (FGU-C) RID, ORCID
Knitlová, Jarmila (FGU-C) RID, ORCID
Lišková, Jana (FGU-C) RID, ORCID
Uhlík, J. (CZ)
Žaloudíková, M. (CZ)
Vondrášek, David (FGU-C) ORCID, SAI
Mikšík, Ivan (FGU-C) RID, ORCID, SAI
Ošťádal, M. (CZ)Source Title Journal of Orthopaedic Research. - : Wiley - ISSN 0736-0266
Roč. 37, č. 3 (2019), s. 769-778Number of pages 10 s. Language eng - English Country GB - United Kingdom Keywords clubfoot ; fibrosis, extracellular matrix ; proteomics ; collagen Subject RIV FI - Traumatology, Orthopedics OECD category Orthopaedics R&D Projects NV17-31564A GA MZd - Ministry of Health (MZ) Method of publishing Limited access Institutional support FGU-C - RVO:67985823 UT WOS 000464419100026 EID SCOPUS 85061451671 DOI 10.1002/jor.24211 Annotation Idiopathic pes equinovarus (clubfoot) is a congenital deformity of the feet and lower legs. Clubfoot belongs to a group of fibro‐proliferative disorders but its origin remains unknown. Our study aimed to achieve the first complex proteomic comparison of clubfoot contracted tissue of the foot (medial side, n = 16), with non‐contracted tissue (lateral side, n = 13). We used label‐free mass spectrometry quantification and immunohistochemistry. Seven proteins were observed to be significantly upregulated in the medial side (asporin, collagen type III, V, and VI, versican, tenascin‐C, and transforming growth factor beta induced protein) and four in the lateral side (collagen types XII and XIV, fibromodulin, and cartilage intermediate layer protein 2) of the clubfoot. Comparison of control samples from cadavers brought only two different protein concentrations (collagen types I and VI). We also revealed pathological calcification and intracellular positivity of transforming growth factor beta only in the contracted tissue of clubfoot. Most of the 11 differently expressed proteins are strongly related to the extracellular matrix architecture and we assume that they may play specific roles in the pathogenesis of this deformity. These proteins seem to be promising targets for future investigations and treatment of this disease. Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2020 Electronic address https://doi.org/10.1002/jor.24211
Number of the records: 1