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HNK-1 in Morphological Study of Development of the Cardiac Conduction System in Selected Groups of Sauropsida
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SYSNO ASEP 0503883 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title HNK-1 in Morphological Study of Development of the Cardiac Conduction System in Selected Groups of Sauropsida Author(s) Kvasilová, A. (CZ)
Gregorovičová, Martina (FGU-C) RID, ORCID, SAI
Kundrát, M. (SK)
Sedmera, David (FGU-C) RID, ORCID, SAISource Title Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology. - : Wiley - ISSN 1932-8486
Roč. 302, č. 1 (2019), s. 69-82Number of pages 14 s. Language eng - English Country US - United States Keywords Leu 7 ; CD 57 ; immunohistochemistry ; heart ; reptile Subject RIV FP - Other Medical Disciplines OECD category Anatomy and morphology (plant science to be 1.6) R&D Projects KJB6111301 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) GA16-02972S GA ČR - Czech Science Foundation (CSF) GA13-12412S GA ČR - Czech Science Foundation (CSF) LTC17023 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LM2015062 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Research Infrastructure Czech-BioImaging - 90062 - Ústav molekulární genetiky AV ČR, v. v. i. Method of publishing Open access Institutional support FGU-C - RVO:67985823 UT WOS 000459881400008 EID SCOPUS 85054860686 DOI 10.1002/ar.23925 Annotation Human natural killer (HNK)-1 antibody is an established marker of developing cardiac conduction system (CCS) in birds and mammals. In our search for the evolutionary origin of the CCS, we tested this antibody in a variety of sauropsid species (Crocodylus niloticus, Varanus indicus, Pogona vitticeps, Pantherophis guttatus, Eublepharis macularius, Gallus gallus, and Coturnix japonica). Hearts of different species were collected at various stages of embryonic development and studied to map immunoreactivity in cardiac tissues. We performed detection on alternating serial paraffin sections using immunohistochemistry for smooth muscle actin or sarcomeric actin as myocardial markers, and HNK-1 to visualize overall staining pattern and then positivity in specific myocyte populations. We observed HNK-1 expression of various intensity distributed in the extracellular matrix and mesenchymal cell surface of cardiac cushions in most of the examined hearts. Strong staining was found in the cardiac nerve fibers and ganglia in all species. The myocardium of the sinus venosus and the atrioventricular canal exhibited transitory patterns of expression. In the Pogona and Crocodylus hearts, as well as in the Gallus and Coturnix ones, additional expression was detected in a subset of myocytes of the (inter)ventricular septum. These results support the use of HNK-1 as a conserved marker of the CCS and suggest that there is a rudimentary CCS present in developing reptilian hearts. Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2020 Electronic address https://doi.org/10.1002/ar.23925
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