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Orientation of nitro-group governs the fluorescence lifetime of nitrobenzoxadiazole (NBD)-labeled lipids in lipid bilayers
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SYSNO ASEP 0503732 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Orientation of nitro-group governs the fluorescence lifetime of nitrobenzoxadiazole (NBD)-labeled lipids in lipid bilayers Author(s) Filipe, H. A. L. (PT)
Pokorná, Šárka (UFCH-W) RID
Hof, Martin (UFCH-W) RID, ORCID
Amaro, Mariana (UFCH-W) RID, ORCID
Loura, L. M. S. (PT)Source Title Physical Chemistry Chemical Physics. - : Royal Society of Chemistry - ISSN 1463-9076
Roč. 21, č. 4 (2019), s. 1682-1688Number of pages 7 s. Language eng - English Country GB - United Kingdom Keywords time-resolved fluorescence ; coarse-grained model ; molecular-dynamics ; force-field ; n-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-labeled lipids ; cell-membranes Subject RIV CF - Physical ; Theoretical Chemistry OECD category Physical chemistry R&D Projects GA17-03160S GA ČR - Czech Science Foundation (CSF) Method of publishing Limited access Institutional support UFCH-W - RVO:61388955 UT WOS 000459584100056 EID SCOPUS 85060401230 DOI 10.1039/c8cp06064a Annotation Nitrobenzoxadiazole (NBD) labeled lipids are popular fluorescent probes of membrane structure and dynamics, and have been widely used in both model systems and living cells. Irrespective of attachment to the lipid head group or hydrocarbon chains, the NBD fluorophore generally adopts a transverse bilayer location near the host lipid carbonyl/glycerol moieties. Still, considerable variability is observed in the measured fluorescence lifetimes, indicating that overall fluorophore location is not the determinant of NBD fluorescence properties. Combining fluorescence experiments and molecular dynamics simulations, we show that for two almost identical NBD probes, significant differences in fluorophore orientation and fluorescence lifetime are observed. Integrating these findings with literature data, we demonstrate a correlation between NBD orientation and fluorescence lifetime. The latter is longer when the NBD nitro group is predominantly oriented towards the bilayer interior, compared to probes for which it points to the water medium. Workplace J. Heyrovsky Institute of Physical Chemistry Contact Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196 Year of Publishing 2020 Electronic address http://hdl.handle.net/11104/0295523
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