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Anterior gradient-3 protein-antibody interaction at charged interfaces. Label-free chronopotentiometric sensing
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SYSNO ASEP 0502570 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Anterior gradient-3 protein-antibody interaction at charged interfaces. Label-free chronopotentiometric sensing Author(s) Ostatná, Veronika (BFU-R) RID, ORCID
Hasoň, Stanislav (BFU-R) RID, ORCID
Kasalová, Veronika (BFU-R) ORCID
Durech, M. (CZ)
Hrstka, R. (CZ)Number of authors 5 Source Title Electrochimica acta. - : Elsevier - ISSN 0013-4686
Roč. 297, FEB 20 2019 (2019), s. 974-979Number of pages 6 s. Publication form Print - P Language eng - English Country GB - United Kingdom Keywords hydrogen evolution ; electrochemistry ; catalysis ; cancer Subject RIV CG - Electrochemistry OECD category Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis) R&D Projects GA18-18154S GA ČR - Czech Science Foundation (CSF) Method of publishing Limited access Institutional support BFU-R - RVO:68081707 UT WOS 000455642500107 DOI 10.1016/j.electacta.2018.12.049 Annotation We developed a fast, simple, label-free method useful for the study of interactions between an antibody (Ab) and antigen based on chronopotentiometric stripping analysis and the catalytic hydrogen evolution reaction. The specific interaction of the Ab, adsorbed at a mercury electrode, with AGR3 protein induced a significant increase in chronopotentiometric peak H in comparison to both the CPS response of the Ab alone and that after incubation with nonspecifically binding proteins. Qualitatively similar results were obtained with another polyclonal antibody specific for AGR2 protein. The demonstrated results, along with previous findings indicate that the proposed technique shows promise as a new option for studying the dynamics, not only of surface-attached antibody-antigen complexes, but also other protein-protein interactions. (C) 2018 Elsevier Ltd. All rights reserved. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2019 Electronic address https://www.sciencedirect.com/science/article/pii/S0013468618327476?via%3Dihub
Number of the records: 1