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Fibroblasts potentiate melanoma cells in vitro invasiveness induced by UV-irradiated keratinocytes
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SYSNO ASEP 0502203 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Fibroblasts potentiate melanoma cells in vitro invasiveness induced by UV-irradiated keratinocytes Author(s) Jobe, N.P. (CZ)
Živicová, V. (CZ)
Mifkova, A. (CZ)
Rosel, D. (CZ)
Dvořánková, B. (CZ)
Kodet, O. (CZ)
Strnad, Hynek (UMG-J) RID
Kolář, Michal (UMG-J) RID, ORCID
Sedo, A. (CZ)
Smetana, K. (CZ)
Strnadova, K. (CZ)
Brabek, J. (CZ)
Lacina, L. (CZ)Number of authors 13 Source Title Histochemistry and Cell Biology. - : Springer - ISSN 0948-6143
Roč. 149, č. 5 (2018), s. 503-516Number of pages 14 s. Language eng - English Country US - United States Keywords Cancer-associated fibroblasts ; Keratinocytes ; Cancer microenvironment ; Cytokine ; Chemokine ; Melanoma Subject RIV EB - Genetics ; Molecular Biology OECD category Biochemistry and molecular biology R&D Projects NV16-29032A GA MZd - Ministry of Health (MZ) LQ1604 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UMG-J - RVO:68378050 UT WOS 000433026100006 DOI 10.1007/s00418-018-1650-4 Annotation Melanoma represents a malignant disease with steadily increasing incidence. UV-irradiation is a recognized key factor in melanoma initiation. Therefore, the efficient prevention of UV tissue damage bears a critical potential for melanoma prevention. In this study, we tested the effect of UV irradiation of normal keratinocytes and their consequent interaction with normal and cancer-associated fibroblasts isolated from melanoma, respectively. Using this model of UV influenced microenvironment, we measured melanoma cell migration in 3-D collagen gels. These interactions were studied using DNA microarray technology, immunofluorescence staining, single cell electrophoresis assay, viability (dead/life) cell detection methods, and migration analysis. We observed that three 10 mJ/cm(2) fractions at equal intervals over 72 h applied on keratinocytes lead to a 50% increase (p < 0.05) in in vitro invasion of melanoma cells. The introduction cancer-associated fibroblasts to such model further significantly stimulated melanoma cells in vitro invasiveness to a higher extent than normal fibroblasts. A panel of candidate gene products responsible for facilitation of melanoma cells invasion was defined with emphasis on IL-6, IL-8, and CXCL-1. In conclusion, this study demonstrates a synergistic effect between cancer microenvironment and UV irradiation in melanoma invasiveness under in vitro condition. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2019
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