Number of the records: 1
Molecular origin of photoprotection in cyanobacteria probed by watermarked femtosecond stimulated Raman spectroscopy
- 1.
SYSNO ASEP 0501677 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Molecular origin of photoprotection in cyanobacteria probed by watermarked femtosecond stimulated Raman spectroscopy Author(s) Hontani, Y. (NL)
Kloz, Miroslav (FZU-D) ORCID
Polívka, T. (CZ)
Shukla, Mahendra K. (MBU-M) RID
Sobotka, Roman (MBU-M) RID, ORCID
Kennis, J.T.M. (NL)Number of authors 6 Source Title Journal of Physical Chemistry Letters. - : American Chemical Society - ISSN 1948-7185
Roč. 9, č. 7 (2018), s. 1788-1792Number of pages 5 s. Language eng - English Country US - United States Keywords light-harvesting complex ; charge-transfer state ; energy-dissipation ; beta-carotene ; transient absorption ; internal-conversion ; excited-states ; protein ; photosynthesis ; mechanism Subject RIV BL - Plasma and Gas Discharge Physics OECD category Fluids and plasma physics (including surface physics) Subject RIV - cooperation Institute of Microbiology - Microbiology, Virology R&D Projects GA17-01137S GA ČR - Czech Science Foundation (CSF) GA17-08755S GA ČR - Czech Science Foundation (CSF) GBP501/12/G055 GA ČR - Czech Science Foundation (CSF) LO1416 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support FZU-D - RVO:68378271 ; MBU-M - RVO:61388971 UT WOS 000429626900050 EID SCOPUS 85045009663 DOI 10.1021/acs.jpclett.8b00663 Annotation Photoprotection is fundamental in photosynthesis to avoid oxidative photodamage upon excess light exposure. Excited chlorophylls (Chl) are quenched by carotenoids, but the precise molecular origin remains controversial. The cyanobacterial HliC protein belongs to the Hlip family ancestral to plant light-harvesting complexes, and binds Chl a and beta-carotene in 2:1 ratio. We analyzed HliC by watermarked femtosecond stimulated Raman spectroscopy to follow the time evolution of its vibrational modes. We observed a 2 ps rise of the C=C stretch band of the 2A(g)(-) (S-1) state of beta- carotene upon CM a excitation, demonstrating energy transfer quenching and fast excess-energy dissipation. We detected two distinct beta-carotene conformers by the C=C stretch frequency of the 2A(g)(-) (S-1) state, but only the beta-carotene whose 2A(g)(-) energy level is significantly lowered and has a lower C=C stretch frequency is involved in quenching. channel.
Workplace Institute of Physics Contact Kristina Potocká, potocka@fzu.cz, Tel.: 220 318 579 Year of Publishing 2019
Number of the records: 1