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Probing the Thermodynamics of Incorporation of N-6-methyl-dATP Opposite an Abasic Site, dCMP, and dTMP During Simulated DNA Synthesis by Differential Scanning Calorimetry
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SYSNO ASEP 0501578 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Probing the Thermodynamics of Incorporation of N-6-methyl-dATP Opposite an Abasic Site, dCMP, and dTMP During Simulated DNA Synthesis by Differential Scanning Calorimetry Author(s) Malina, Jaroslav (BFU-R) ORCID
Brabec, Viktor (BFU-R) RID, ORCIDNumber of authors 2 Source Title ChemistrySelect. - : Wiley - ISSN 2365-6549
Roč. 3, č. 46 (2018), s. 13076-13080Number of pages 5 s. Publication form Print - P Language eng - English Country DE - Germany Keywords polymerase ; damage ; nucleoside ; energetics Subject RIV CE - Biochemistry OECD category Biochemistry and molecular biology R&D Projects GA17-09436S GA ČR - Czech Science Foundation (CSF) Institutional support BFU-R - RVO:68081707 UT WOS 000453576900014 DOI 10.1002/slct.201803565 Annotation Previous reports indicated that when an abasic (apurinic/apyrimidinic, AP) site is bypassed by DNA polymerases, dATP is preferentially inserted. Here we evaluate, using differential scanning calorimetry, the thermodynamic changes associated with incorporation of N-6-methyl-dATP opposite an AP site, dCMP, and dTMP during simulated DNA polymerization. The results confirm that AP sites block DNA polymerases one nucleotide prior to the lesion. Thermodynamic data imply that the propensity of N-6-methyl-dAMP for elongation, when incorporated opposite an AP site, is higher than that of dAMP in agreement with a higher promutagenic potential of N-6-methyl-dATP if placed opposite a non-instructional DNA lesion, such as an AP site. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2019
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