Number of the records: 1

Cryo-SEM of Cell Cortex Architecture and Membrane Ultrastructure in Eugregarines

SYSNO ASEP	0501298
Document Type	A - Abstract
R&D Document Type	The record was not marked in the RIV
R&D Document Type	Není vybrán druh dokumentu
Title	Cryo-SEM of Cell Cortex Architecture and Membrane Ultrastructure in Eugregarines
Author(s)	Vaškovicová, Naděžda (UPT-D)_{RID, ORCID, SAI} Kováčiková, M. (CZ) Valigurová, A. (CZ) Diakin, A. (CZ) Krzyžánek, Vladislav (UPT-D)_{RID, ORCID, SAI}
Number of authors	5
Source Title	Mikroskopie 2018. - Praha : Československá mikroskopická společnost, 2018 S. 76
Number of pages	1 s.
Publication form	Print - P
Action	Mikroskopie 2018
Event date	14.05.2018 - 16.05.2018
VEvent location	Lednice na Moravě
Country	CZ - Czech Republic
Event type	EUR
Language	eng - English
Country	CZ - Czech Republic
Keywords	Cryo-SEM ; Eugregarines ; freeze-fracture ; filamentous structures ; cytoplasmic organelles
Subject RIV	JA - Electronics ; Optoelectronics, Electrical Engineering
OECD category	Electrical and electronic engineering
R&D Projects	GA17-15451S GA ČR - Czech Science Foundation (CSF)
	LO1212 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	ED0017/01/01 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	UPT-D - RVO:68081731
Annotation	Cryo-SEM of frozen microscopic structures, especially unicellular organisms, is a fast method for processing the sample for electron microscopy. Cells suspension in buffer were frozen in the high-pressure-freezing device HPM 100 (Leica microsystems) on 3 mm carriers and placed inside the cryo-high-vacuum chamber ACE 600 (Leica microsystems). Cells were fractured and after a short etching transferred into the SEM Magellan 400 (FEI) by vacuum shuttle VCT100 (Leica microsystems). The cryo-SEM analysis was performed at temperature of -120°C, electron probe current 3.1-6.3pA, and electron energy 1-2keV.
Workplace	Institute of Scientific Instruments
Contact	Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178
Year of Publishing	2019

Number of the records: 1