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Poly(D,L-lactide)/polyethylene glycol micro/nanofiber mats as paclitaxel-eluting carriers: preparation and characterization of fibers, in vitro drug release, antiangiogenic activity and tumor recurrence prevention

SYSNO ASEP	0500120
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Poly(D,L-lactide)/polyethylene glycol micro/nanofiber mats as paclitaxel-eluting carriers: preparation and characterization of fibers, in vitro drug release, antiangiogenic activity and tumor recurrence prevention
Author(s)	Hobzová, Radka (UMCH-V)_{RID, ORCID} Hampejsová, Z. (CZ) Černá, T. (CZ) Hraběta, J. (CZ) Venclíková, Kristýna (UMCH-V)_RID Jedelská, J. (DE) Bakowsky, U. (DE) Bosáková, Z. (CZ) Lhotka, M. (CZ) Vaculín, Š. (CZ) Franěk, M. (CZ) Steinhart, Miloš (UMCH-V)_RID Kovářová, Jana (UMCH-V)_RID Michálek, Jiří (UMCH-V)_{RID, ORCID} Širc, Jakub (UMCH-V)_{RID, ORCID}
Source Title	Materials Science & Engineering C-Materials for Biological Applications. - : Elsevier - ISSN 0928-4931 Roč. 98, May (2019), s. 982-993
Number of pages	12 s.
Language	eng - English
Country	NL - Netherlands
Keywords	PLA/PEG micro/nanofibers ; needleless electrospinning ; paclitaxel quantification
Subject RIV	CD - Macromolecular Chemistry
OECD category	Polymer science
R&D Projects	GA16-04863S GA ČR - Czech Science Foundation (CSF)
	LQ1604 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Method of publishing	Limited access
Institutional support	UMCH-V - RVO:61389013
UT WOS	000461526800097
EID SCOPUS	85060105949
DOI	10.1016/j.msec.2019.01.046
Annotation	Poly(d,l-lactide)/polyethylene glycol (PLA/PEG) micro/nanofibers loaded with paclitaxel (PTX, 10 wt%) were prepared by needless electrospinning technology, which allows large scale production for real medicinal practice. The fiber structure and properties were investigated by several methods including scanning electron microscopy, nitrogen adsorption/desorption isotherm measurements, differential scanning calorimetry, and X-ray diffraction measurements to examine their morphology (fiber diameter distribution, specific surface area, and total pore volume), composition, drug-loading efficiency, and physical state. An HPLC-UV method was optimized and validated to quantify in vitro PTX release into PBS. The results showed that the addition of PEG into PLA fibers promoted the release of higher amounts of hydrophobic PTX over prolonged time periods compared to fibers without PEG. An in vitro cell assay demonstrated the biocompatibility of PLA/PEG fibrous materials and showed significant cytotoxicity of PTX-loaded PLA/PEG fibers against a human fibrosarcoma HT1080 cell line. The chick chorioallantoic membrane assay proved that PTX-loaded fibers exhibited antiangiogenic activity, with a pronounced effect in the case of the PEG-containing fibers. In vivo evaluation of PTX-loaded PLA/PEG fibers in a human fibrosarcoma recurrence model showed statistically significant inhibition in tumor incidence and growth after primary tumor resection compared to other treatment groups.
Workplace	Institute of Macromolecular Chemistry
Contact	Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358
Year of Publishing	2020
Electronic address	https://www.sciencedirect.com/science/article/pii/S0928493118308683?via%3Dihub

Number of the records: 1