Unravelling species boundaries in the Aspergillus viridinutans complex (section Fumigati): opportunistic human and animal pathogens capable of interspecific hybridization

Hubka, Vít; Barrs, V.; Dudová, Z.; Sklenář, František; Kubátová, A.; Matsuzawa, T.; Yaguchi, T.; Horie, Y.; Nováková, Alena; Frisvad, J.C.; Talbot, J.J.; Kolařík, Miroslav

doi:https://dx.doi.org/10.3767/persoonia.2018.41.08

Number of the records: 1

Unravelling species boundaries in the Aspergillus viridinutans complex (section Fumigati): opportunistic human and animal pathogens capable of interspecific hybridization

1.

SYSNO ASEP	0499826
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Unravelling species boundaries in the Aspergillus viridinutans complex (section Fumigati): opportunistic human and animal pathogens capable of interspecific hybridization
Author(s)	Hubka, Vít (MBU-M)_ORCID Barrs, V. (AU) Dudová, Z. (CZ) Sklenář, František (MBU-M)_ORCID Kubátová, A. (CZ) Matsuzawa, T. (JP) Yaguchi, T. (JP) Horie, Y. (JP) Nováková, Alena (MBU-M)_ORCID Frisvad, J.C. (DK) Talbot, J.J. (AU) Kolařík, Miroslav (MBU-M)_{RID, ORCID}
Source Title	Persoonia. - : Naturalis Biodiversity Center - ISSN 0031-5850 Roč. 41, DEC 2018 (2018), s. 142-174
Number of pages	33 s.
Language	eng - English
Country	NL - Netherlands
Keywords	Aspergillus felis ; Aspergillus fumigatus ; invasive aspergillosis
Subject RIV	EE - Microbiology, Virology
OECD category	Microbiology
R&D Projects	ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	GA17-20286S GA ČR - Czech Science Foundation (CSF)
Institutional support	MBU-M - RVO:61388971
UT WOS	000454875300008
DOI	10.3767/persoonia.2018.41.08
Annotation	Although Aspergillus fumigatus is the major agent of invasive aspergillosis, an increasing number of infections are caused by its cryptic species, especially A. lentulus and the A. viridinutans species complex (AVSC).Their identification is clinically relevant because of antifungal drug resistance and refractory infections. Species boundaries in the AVSC are unresolved since most species have uniform morphology and produce interspecific hybrids in vitro. Clinical and environmental strains from six continents (n = 110) were characterized by DNA sequencing of four to six loci. Biological compatibilities were tested within and between major phylogenetic clades, and ascospore morphology was characterised. Species delimitation methods based on the multispecies coalescent model (MSC) supported recognition of ten species including one new species. Four species are confirmed opportunistic pathogens, A. udagawae followed by A. felis and A. pseudoviridinutans are known from opportunistic human infections, while A. felis followed by A. udagawae and A. wyomingensis are agents of feline sino-orbital aspergillosis. Recently described human-pathogenic species A. parafelis and A. pseudofelis are synonymized with A. felis and an epitype is designated for A. udagawae. Intraspecific mating assay showed that only a few of the heterothallic species can readily generate sexual morphs in vitro. Interspecific mating assays revealed that five different species combinations were biologically compatible. Hybrid ascospores had atypical surface ornamentation and significantly different dimensions compared to parental species. This suggests that species limits in theAVSC are maintained by both pre- and post-zygotic barriers and these species display a great potential for rapid adaptation and modulation of virulence.
Workplace	Institute of Microbiology
Contact	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Year of Publishing	2019

Number of the records: 1