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Muscle Cell and Tissue : Current Status of Research Field

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    SYSNO ASEP0499717
    Document TypeM - Monograph Chapter
    R&D Document TypeMonograph Chapter
    TitleVascular Smooth Muscle Cells (VSMCs) in Blood Vessel Tissue Engineering: The Use of Differentiated Cells or Stem Cells as VSMC Precursors
    Author(s) Bačáková, Lucie (FGU-C) RID, ORCID
    Trávníčková, Martina (FGU-C) RID, ORCID, SAI
    Filová, Elena (FGU-C) RID
    Matějka, Roman (FGU-C) RID, ORCID, SAI
    Štěpanovská, Jana (FGU-C) ORCID, RID
    Musílková, Jana (FGU-C) RID, ORCID
    Zárubová, Jana (FGU-C) RID, ORCID
    Molitor, M. (CZ)
    Source TitleMuscle Cell and Tissue : Current Status of Research Field. - London : IntechOpen, 2018 / Sakuma Kunihiro - ISBN 978-1-78984-006-3
    Pagess. 289-308
    Number of pages20 s.
    Number of pages357
    Publication formOnline - E
    Languageeng - English
    CountryGB - United Kingdom
    Keywordsvascular replacement ; adipose tissue-derived stem cells ; transforming growth factor-beta ; bone morphogenetic protein-4 ; mechanical loading ; dynamic bioreactors ; smooth muscle cell differentiation ; tissue engineering ; regenerative medicine
    Subject RIVEI - Biotechnology ; Bionics
    OECD categoryTechnologies involving the manipulation of cells, tissues, organs or the whole organism (assisted reproduction)
    R&D ProjectsNV15-33018A GA MZd - Ministry of Health (MZ)
    LQ1604 GA MŠk - Ministry of Education, Youth and Sports (MEYS)
    LM2015062 GA MŠk - Ministry of Education, Youth and Sports (MEYS)
    Institutional supportFGU-C - RVO:67985823
    AnnotationVascular smooth muscle cells (VSMCs) play important roles in the physiology and pathophysiology of the blood vessels. In a healthy adult organism, VSMCs are quiescent, but after a blood vessel injury, they undergo phenotypic modulation from the contractile phenotype to the synthetic phenotype, characterized by high activity in migration, proliferation and proteosynthesis. This behavior of VSMCs can lead to stenosis or obliteration of the vascular lumen. For this reason, VSMCs have tended to be avoided in the construction of blood vessel replacements. However, VSMCs are a physiological and the most numerous component of blood vessels, so their presence in novel advanced vascular replacements is indispensable. Either differentiated VSMCs or stem cells as precursors of VSMCs can be used in the reconstruction of the tunica media in these replacements. VSMCs can be obtained from blood vessels (usually from subcutaneous veins) taken surgically from the patients and can be expanded in vitro. During in vitro cultivation, VSMCs lose their differentiation markers, at least partly. These cells should therefore be re-differentiated by seeding them on appropriate scaffolds by composing cell culture media and by mechanical stimulation in dynamic bioreactors. Similar approaches can also be applied for differentiating stem cells, particularly adipose tissue-derived stem cells, toward VSMCs for the purposes of vascular tissue engineering.
    WorkplaceInstitute of Physiology
    ContactLucie Trajhanová,, Tel.: 241 062 400
    Year of Publishing2019
Number of the records: 1