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Muscle Cell and Tissue : Current Status of Research Field
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SYSNO ASEP 0499717 Document Type M - Monograph Chapter R&D Document Type Monograph Chapter Title Vascular Smooth Muscle Cells (VSMCs) in Blood Vessel Tissue Engineering: The Use of Differentiated Cells or Stem Cells as VSMC Precursors Author(s) Bačáková, Lucie (FGU-C) RID, ORCID
Trávníčková, Martina (FGU-C) RID, ORCID, SAI
Filová, Elena (FGU-C) RID, ORCID
Matějka, Roman (FGU-C) RID, ORCID, SAI
Štěpanovská, Jana (FGU-C) ORCID, RID
Musílková, Jana (FGU-C) RID, ORCID
Zárubová, Jana (FGU-C) RID, ORCID
Molitor, M. (CZ)Source Title Muscle Cell and Tissue : Current Status of Research Field. - London : IntechOpen, 2018 / Sakuma Kunihiro - ISBN 978-1-78984-006-3 Pages s. 289-308 Number of pages 20 s. Number of pages 357 Publication form Online - E Language eng - English Country GB - United Kingdom Keywords vascular replacement ; adipose tissue-derived stem cells ; transforming growth factor-beta ; bone morphogenetic protein-4 ; mechanical loading ; dynamic bioreactors ; smooth muscle cell differentiation ; tissue engineering ; regenerative medicine Subject RIV EI - Biotechnology ; Bionics OECD category Technologies involving the manipulation of cells, tissues, organs or the whole organism (assisted reproduction) R&D Projects NV15-33018A GA MZd - Ministry of Health (MZ) LQ1604 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LM2015062 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support FGU-C - RVO:67985823 DOI 10.5772/intechopen.77108 Annotation Vascular smooth muscle cells (VSMCs) play important roles in the physiology and pathophysiology of the blood vessels. In a healthy adult organism, VSMCs are quiescent, but after a blood vessel injury, they undergo phenotypic modulation from the contractile phenotype to the synthetic phenotype, characterized by high activity in migration, proliferation and proteosynthesis. This behavior of VSMCs can lead to stenosis or obliteration of the vascular lumen. For this reason, VSMCs have tended to be avoided in the construction of blood vessel replacements. However, VSMCs are a physiological and the most numerous component of blood vessels, so their presence in novel advanced vascular replacements is indispensable. Either differentiated VSMCs or stem cells as precursors of VSMCs can be used in the reconstruction of the tunica media in these replacements. VSMCs can be obtained from blood vessels (usually from subcutaneous veins) taken surgically from the patients and can be expanded in vitro. During in vitro cultivation, VSMCs lose their differentiation markers, at least partly. These cells should therefore be re-differentiated by seeding them on appropriate scaffolds by composing cell culture media and by mechanical stimulation in dynamic bioreactors. Similar approaches can also be applied for differentiating stem cells, particularly adipose tissue-derived stem cells, toward VSMCs for the purposes of vascular tissue engineering. Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2019
Number of the records: 1