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Nanofiber dressing consisting of antisense rna-functionalized nanodiamonds for therapy of non-healing wounds in diabetic individuals
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SYSNO ASEP 0499508 Document Type C - Proceedings Paper (int. conf.) R&D Document Type Conference Paper Title Nanofiber dressing consisting of antisense rna-functionalized nanodiamonds for therapy of non-healing wounds in diabetic individuals Author(s) Neuhoferová, Eva (MBU-M) ORCID
Petráková, V. (CZ)
Vocetková, K. (CZ)
Kindermann, Marek (MBU-M)
Křivohlavá, Romana (MBU-M) RID
Benson, Veronika (MBU-M) RID, ORCIDSource Title CONFERENCE PROCEEDINGS NANOCON 2017. - Brno : Tnager Ltd., 2018 - ISBN 9788087294819 Pages s. 467-472 Number of pages 6 s. Publication form Print - P Action 9th International Conference on Nanomaterials - Research and Application (NANOCON) Event date 18.10.2017 - 20.10.2017 VEvent location Brno Country CZ - Czech Republic Event type WRD Language eng - English Country CZ - Czech Republic Keywords Electrospun nanofibers ; fluorescent nanodiamonds ; RNA interference Subject RIV EE - Microbiology, Virology OECD category Microbiology R&D Projects NV15-33094A GA MZd - Ministry of Health (MZ) Institutional support MBU-M - RVO:61388971 UT WOS 000452823300076 EID SCOPUS 85052321107 Annotation Non-healing wounds are serious complication in diabetic patients and represent an attractive challenge for development of suitable carrier system possessing constant and localized release of therapeutic biomolecule into the wound without any undesired side effects. Given the fact that these non-healing wounds are result of impaired balance in metalloproteinases synthesized by immune cells residing the wounds, gene therapy offering knock down of such enzymes is of great interest.
Here we challenged a development of functional and biocompatible wound dressing enabling controlled release of trackable carrier loaded with therapeutic siRNA. Our dressing consists of scaffold from degradable polymer nanofibers enriched with fluorescent nanodiamond particles (FND). We have previously shown the nanodiamond particles are great carriers for antisense RNAs. Their advantages represent high biocompatibility, stable luminescence giving us the possibility to track the carrier system in the wound, and effective release of antisense RNA in the wound. Embedding of nanodiamond-siRNA systems into nanofiber scaffold enables continuous release of siRNA and maintaining the stable siRNA concentration in the wound site resulting in a promotion of wound healing.
We developed FND-siRNA complexes specific to MMP-9 that efficiently inhibit the expression of target MMP-9 mRNA. The complexes were embedded into core/shell nanofibers from PVA and PCL, visualized by confocal microscopy, and characterized by electron microscopy. Real-time PCR was used to assess the silencing effect of siRNA that has been delivered to target murine fibroblasts by FND released from nanofiber dressing. Nanofiber system with embedded FNDs was applied on wounds in diabetic animal models to evaluate its suitability regarding short and long term toxicity, efficacy, and handling in vivo.
Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2019
Number of the records: 1