Number of the records: 1

Metabarcoding analysis of strongylid nematode diversity in two sympatric primate species

SYSNO ASEP	0498784
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Metabarcoding analysis of strongylid nematode diversity in two sympatric primate species
Author(s)	Pafčo, B. (CZ) Čížková, D. (CZ) Kreisinger, J. (CZ) Hasegawa, H. (JP) Vallo, P. (CZ) Shutt, K. (GB) Todd, A. (CF) Petrželková, Klára Judita (BC-A)_RID Modrý, David (BC-A)_{RID, ORCID}
Number of authors	9
Article number	5933
Source Title	Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322 Roč. 8, APR 12 (2018)
Number of pages	11 s.
Publication form	Online - E
Language	eng - English
Country	GB - United Kingdom
Keywords	WESTERN LOWLAND GORILLAS ; OESOPHAGOSTOMUM-BIFURCUM NEMATODA ; INTERNAL TRANSCRIBED SPACER ; NECATOR-AMERICANUS
Subject RIV	GJ - Animal Vermins ; Diseases, Veterinary Medicine
OECD category	Veterinary science
Institutional support	BC-A - RVO:60077344
UT WOS	000429785900073
EID SCOPUS	85045440671
DOI	10.1038/s41598-018-24126-3
Annotation	Strongylid nematodes in large terrestrial herbivores such as great apes, equids, elephants, and humans tend to occur in complex communities. However, identification of all species within strongylid communities using traditional methods based on coproscopy or single nematode amplification and sequencing is virtually impossible. High-throughput sequencing (HTS) technologies provide opportunities to generate large amounts of sequence data and enable analyses of samples containing a mixture of DNA from multiple species/genotypes. We designed and tested an HTS approach for strain-level identification of gastrointestinal strongylids using ITS-2 metabarcoding at the MiSeq Illumina platform in samples from two free-ranging non-human primate species inhabiting the same environment, but differing significantly in their host traits and ecology. Although we observed overlapping of particular haplotypes, overall the studied primate species differed in their strongylid nematode community composition. Using HTS, we revealed hidden diversity in the strongylid nematode communities in non-human primates, more than one haplotype was found in more than 90% of samples and coinfections of more than one putative species occurred in 80% of samples. In conclusion, the HTS approach on strongylid nematodes, preferably using fecal samples, represents a time and cost-efficient way of studying strongylid communities and provides a resolution superior to traditional approaches.
Workplace	Biology Centre (since 2006)
Contact	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Year of Publishing	2019
Electronic address	https://www.nature.com/articles/s41598-018-24126-3

Number of the records: 1