Number of the records: 1
Asymmetric distribution of biomolecules of maternal origin in the Xenopus laevis egg and their impact on the developmental plan
- 1.
SYSNO ASEP 0496977 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Asymmetric distribution of biomolecules of maternal origin in the Xenopus laevis egg and their impact on the developmental plan Author(s) Šindelka, Radek (BTO-N) RID
Abaffy, Pavel (BTO-N)
Qu, Y. (US)
Tománková, Silvie (BTO-N) RID
Šídová, Monika (BTO-N) RID
Naraine, Ravindra (BTO-N)
Kolář, Michal (UMG-J) RID, ORCID
Peuchen, E. (US)
Sun, L. (US)
Dovichi, N. (US)
Kubista, Mikael (BTO-N) RIDNumber of authors 11 Article number 8315 Source Title Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322
Roč. 8, MAY 29 2018 (2018)Number of pages 16 s. Language eng - English Country GB - United Kingdom Keywords CELL MASS-SPECTROMETRY ; BICOID MESSENGER-RNA ; SAMPLE PREPARATION Subject RIV EB - Genetics ; Molecular Biology OECD category Other biological topics Subject RIV - cooperation Institute of Molecular Genetics - Genetics ; Molecular Biology R&D Projects GA16-07500S GA ČR - Czech Science Foundation (CSF) LH15074 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LM2015047 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support BTO-N - RVO:86652036 ; UMG-J - RVO:68378050 UT WOS 000433291300018 EID SCOPUS 85047852101 DOI 10.1038/s41598-018-26592-1 Annotation Asymmetric cell division is a ubiquitous feature during the development of higher organisms. Asymmetry is achieved by differential localization or activities of biological molecules such as proteins, and coding and non-coding RNAs. Here, we present subcellular transcriptomic and proteomic analyses along the animal-vegetal axis of Xenopus laevis eggs. More than 98% of the maternal mRNAs could be categorized into four localization profile groups: animal, vegetal, extremely vegetal, and a newly described group of mRNAs that we call extremely animal, which are mRNAs enriched in the animal cortex region. 3'UTRs of localized mRNAs were analyzed for localization motifs. Several putative motifs were discovered for vegetal and extremely vegetal mRNAs, while no distinct conserved motifs for the extremely animal mRNAs were identified, suggesting different localization mechanisms. Asymmetric profiles were also found for proteins, with correlation to those of corresponding mRNAs. Based on unexpected observation of the profiles of the homoeologous genes exd2 we propose a possible mechanism of genetic evolution. Workplace Institute of Biotechnology Contact Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Year of Publishing 2019
Number of the records: 1