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The fibroblast surface markers FAP, anti-fibroblast, and FSP are expressed by cells of epithelial origin and may be altered during epithelial-to-mesenchymal transition
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SYSNO ASEP 0495092 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The fibroblast surface markers FAP, anti-fibroblast, and FSP are expressed by cells of epithelial origin and may be altered during epithelial-to-mesenchymal transition Author(s) Kahounová, Zuzana (BFU-R) ORCID
Kurfurstova, D. (CZ)
Bouchal, J. (CZ)
Kharaishvili, G. (CZ)
Navrátil, J. (CZ)
Remšík, Jan (BFU-R)
Šimečková, Šárka (BFU-R) ORCID
Študent, V. (CZ)
Kozubík, Alois (BFU-R) RID, ORCID
Souček, Karel (BFU-R) RID, ORCIDNumber of authors 10 Source Title Cytometry. Part A. - : Wiley - ISSN 1552-4922
Roč. 93A, č. 9 (2018), s. 941-951Number of pages 11 s. Publication form Print - P Language eng - English Country US - United States Keywords cancer-associated fibroblasts ; prostate-cancer ; activation protein ; rock inhibitor ; ikk-beta ; breast ; growth ; transformation ; model ; differentiation Subject RIV EB - Genetics ; Molecular Biology OECD category Cell biology R&D Projects GA15-11707S GA ČR - Czech Science Foundation (CSF) NV15-33999A GA MZd - Ministry of Health (MZ) NV15-28628A GA MZd - Ministry of Health (MZ) 7AMB16AT022 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support BFU-R - RVO:68081707 UT WOS 000445603300008 EID SCOPUS 85017381653 DOI 10.1002/cyto.a.23101 Annotation The identification of fibroblasts and cancer-associated fibroblasts from human cancer tissue using surface markers is difficult, especially because the markers used currently are usually not expressed solely by fibroblasts, and the identification of fibroblast-specific surface molecules is still under investigation. It was aimed to compare three commercially available antibodies in the detection of different surface epitopes of fibroblasts (anti-fibroblast, fibroblast activation protein , and fibroblast surface protein). The specificity of their expression, employing fibroblast cell lines and tumor-derived fibroblasts from breast and prostate tissues was investigated. Both the established fibroblast cell line HFF-1 and ex vivo primary fibroblasts isolated from breast and prostate cancer tissues expressed the tested surface markers to different degrees. Surprisingly, those markers were expressed also by permanent cell lines of epithelial origin, both benign and cancer-derived (breast-cell lines MCF 10A, HMLE and prostate-cell lines BPH-1, DU 145, and PC-3). The expression of fibroblast activation protein increased on the surface of previously described models of epithelial cells undergoing epithelial-to-mesenchymal transition in response to treatment with TGF-1. To prove the co-expression of the fibroblast markers on cells of epithelial origin, we used freshly dissociated human prostate and breast cancer tissues. The results confirmed the co-expression of anti-fibroblast and fibroblast surface protein on CD31/CD45-negative/EpCAM-positive epithelial cells. In summary, our data support the findings that the tested fibroblast markers are not fibroblast specific and may be expressed also by cells of epithelial origin (e.g., cells undergoing EMT). Therefore, the expression of these markers should be interpreted with caution, and the combination of several epitopes for both positive (anti-fibroblast or fibroblast activation protein ) and negative (EpCAM) identification of fibroblasts from breast and prostate tumor tissues is advised. (c) 2017 International Society for Advancement of Cytometry Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2019 Electronic address https://onlinelibrary.wiley.com/doi/10.1002/cyto.a.23101
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