Chromatin architecture changes and DNA replication fork collapse are critical features in cryopreserved cells that are differentially controlled by cryoprotectants

Falk, Martin; Falková, Iva; Kopečná, Olga; Bačíková, Alena; Pagáčová, Eva; Šimek, Daniel; Golan, Martin; Kozubek, Stanislav; Pekarová, Michaela; Follett, S.E.; Klejdus, B.; Elliott, K.W.; Varga, K.; Teplá, O.; Kratochvílová, Irena

doi:https://dx.doi.org/10.1038/s41598-018-32939-5

Number of the records: 1

Chromatin architecture changes and DNA replication fork collapse are critical features in cryopreserved cells that are differentially controlled by cryoprotectants

1.

SYSNO ASEP	0494984
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Chromatin architecture changes and DNA replication fork collapse are critical features in cryopreserved cells that are differentially controlled by cryoprotectants
Author(s)	Falk, Martin (BFU-R)_{RID, ORCID} Falková, Iva (BFU-R)_ORCID Kopečná, Olga (BFU-R)_ORCID Bačíková, Alena (BFU-R) Pagáčová, Eva (BFU-R)_ORCID Šimek, Daniel (FZU-D)_{RID, ORCID} Golan, Martin (FZU-D)_RID Kozubek, Stanislav (BFU-R)_RID Pekarová, Michaela (BFU-R)_RID Follett, S.E. (US) Klejdus, B. (CZ) Elliott, K.W. (US) Varga, K. (US) Teplá, O. (CZ) Kratochvílová, Irena (FZU-D)_{RID, ORCID, SAI}
Number of authors	15
Article number	14694
Source Title	Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322 Roč. 8, OCT 2 2018 (2018)
Number of pages	18 s.
Publication form	Online - E
Language	eng - English
Country	GB - United Kingdom
Keywords	human spermatozoa ; oxidative-stress ; damage response ; repair ; trehalose ; integrity ; dynamics ; sperm ; fragmentation ; instability
Subject RIV	BO - Biophysics
OECD category	Cell biology
Subject RIV - cooperation	Institute of Physics - Biophysics
R&D Projects	NV16-29835A GA MZd - Ministry of Health (MZ)
	GA16-12454S GA ČR - Czech Science Foundation (CSF)
	LO1409 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	BFU-R - RVO:68081707 ; FZU-D - RVO:68378271
UT WOS	000446035900024
EID SCOPUS	85054195372
DOI	10.1038/s41598-018-32939-5
Annotation	In this work, we shed new light on the highly debated issue of chromatin fragmentation in cryopreserved cells. Moreover, for the first time, we describe replicating cell-specific DNA damage and higher-order chromatin alterations after freezing and thawing. We identified DNA structural changes associated with the freeze-thaw process and correlated them with the viability of frozen and thawed cells. We simultaneously evaluated DNA defects and the higher-order chromatin structure of frozen and thawed cells with and without cryoprotectant treatment. We found that in replicating (S phase) cells, DNA was preferentially damaged by replication fork collapse, potentially leading to DNA double strand breaks (DSBs), which represent an important source of both genome instability and defects in epigenome maintenance. This induction of DNA defects by the freeze-thaw process was not prevented by any cryoprotectant studied. Both in replicating and non-replicating cells, freezing and thawing altered the chromatin structure in a cryoprotectant-dependent manner. Interestingly, cells with condensed chromatin, which was strongly stimulated by dimethyl sulfoxide (DMSO) prior to freezing had the highest rate of survival after thawing. Our results will facilitate the design of compounds and procedures to decrease injury to cryopreserved cells.
Workplace	Institute of Biophysics
Contact	Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244
Year of Publishing	2019

Number of the records: 1