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Repetitive somatic embryogenesis induced cytological and proteomic changes in embryogenic lines of Pseudotsuga menziesii [Mirb.]
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SYSNO ASEP 0493934 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Repetitive somatic embryogenesis induced cytological and proteomic changes in embryogenic lines of Pseudotsuga menziesii [Mirb.] Author(s) Gautier, F. (FR)
Eliášová, Kateřina (UEB-Q) RID, ORCID
Leple, J.C. (FR)
Vondráková, Zuzana (UEB-Q) RID, ORCID
Lomenech, A. M. (FR)
Le Metté, C. (FR)
Label, P. (FR)
Costa, G. (PT)
Trontin, J.F. (FR)
Teyssier, C. (FR)
Lelu-Walter, M.A. (FR)Number of authors 11 Article number 164 Source Title BMC Plant Biology. - : BioMed Central - ISSN 1471-2229
Roč. 18, AUG 10 (2018)Number of pages 24 s. Language eng - English Country GB - United Kingdom Keywords programmed cell-death ; pinus-pinaster ait. ; norway spruce ; zygotic embryos ; maritime pine ; picea-abies ; salicylic-acid ; abiotic stress ; abscisic-acid ; larix ; Douglas-fir ; Embryogenic potential ; Proliferation ; Proteomic ; Cytology ; Plant growth regulators Subject RIV EF - Botanics OECD category Plant sciences, botany R&D Projects LTC17030 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UEB-Q - RVO:61389030 UT WOS 000441272500004 EID SCOPUS 85051540656 DOI 10.1186/s12870-018-1337-y Annotation To explore poorly understood differences between primary and subsequent somatic embryogenic lines of plants, we induced secondary (2ry) and tertiary (3ry) lines from cotyledonary somatic embryos (SEs) of two Douglas-fir genotypes: SD4 and TD17. The 2ry lines exhibited significantly higher embryogenic potential (SE yields) than the 1ry lines initiated from zygotic embryos (SD4, 2155 vs 477, TD17, 240 vs 29 g(-1) f.w.). Moreover, we observed similar differences in yield between 2ry and 3ry lines of SD4 (2400 vs 3921 g(-1) f.w.). To elucidate reasons for differences in embryogenic potential induced by repetitive somatic embryogenesis we then compared 2ry vs 1ry and 2ry vs 3ry lines at histo-cytological (using LC-MS/MS) and proteomic levels. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2019
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