Number of the records: 1
Differential modulation of white adipose tissue endocannabinoid levels by n-3 fatty acids in obese mice and type 2 diabetic patients
- 1.
SYSNO ASEP 0493048 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Differential modulation of white adipose tissue endocannabinoid levels by n-3 fatty acids in obese mice and type 2 diabetic patients Author(s) Rossmeisl, Martin (FGU-C) RID, ORCID
Pavlišová, Jana (FGU-C)
Janovská, Petra (FGU-C) RID, ORCID
Kuda, Ondřej (FGU-C) RID, ORCID, SAI
Bardová, Kristina (FGU-C) RID, ORCID, SAI
Hansíková, Jana (FGU-C)
Svobodová, Michaela (FGU-C) RID, ORCID
Oseeva, Marina (FGU-C) ORCID, RID
Veleba, J. (CZ)
Kopecký Jr., J. (CZ)
Žáček, P. (CZ)
Fišerová, E. (CZ)
Pelikánová, T. (CZ)
Kopecký, Jan (FGU-C) RID, ORCIDSource Title Biochimica Et Biophysica Acta-Molecular and Cell Biology of Lipids. - : Elsevier - ISSN 1388-1981
Roč. 1863, č. 7 (2018), s. 712-725Number of pages 14 s. Language eng - English Country NL - Netherlands Keywords 2-AG ; DHEA ; high-fat diet ; obesity ; Omega-3 PUFA Subject RIV FB - Endocrinology, Diabetology, Metabolism, Nutrition OECD category Endocrinology and metabolism (including diabetes, hormones) R&D Projects GA14-09347S GA ČR - Czech Science Foundation (CSF) NV15-27431A GA MZd - Ministry of Health (MZ) Institutional support FGU-C - RVO:67985823 UT WOS 000444364300005 EID SCOPUS 85045450551 DOI 10.1016/j.bbalip.2018.03.011 Annotation n-3 polyunsaturated fatty acids (n-3 PUFA) might regulate metabolism by lowering endocannabinoid levels. We examined time-dependent changes in adipose tissue levels of endocannabinoids as well as in parameters of glucose homeostasis induced by n-3 PUFA in dietary-obese mice, and compared these results with the effect of n-3 PUFA intervention in type 2 diabetic (T2DM) subjects. Male C57BL/6J mice were fed for 8, 16 or 24 weeks a high-fat diet alone (cHF) or supplemented with n-3 PUFA (cHF + F). Overweight/obese, T2DM patients on metformin therapy were given for 24 weeks corn oil (Placebo, 5 g/day) or n-3 PUFA concentrate as above (Omega-3, 5 g/day). Endocannabinoids were measured by liquid chromatography-tandem mass-spectrometry. Compared to cHF-fed controls, the cHF + F mice consistently reduced 2-arachidonoylglycerol (up to similar to 2-fold at week 24) and anandamide (similar to 2-fold) in adipose tissue, while the levels of endocannabinoid-related anti-inflammatory molecules N-eicosapentaenoyl ethanolamine (EPEA) and N-docosahexaenoyl ethanolamine (DHEA) increased more than similar to 10-fold and similar to 8-fold, respectively. At week 24, the cHF + F mice improved glucose tolerance and fasting blood glucose, the latter being positively correlated with adipose 2-arachidonoylglycerol levels only in obese cHF-fed controls, like fasting insulin and HOMA-IR. In the patients, n-3 PUFA failed to reduce 2-arachidonoylglycerol and anandamide levels in adipose tissue and serum, but they increased both adipose tissue and serum levels of EPEA and DHEA. In conclusion, the inability of n-3 PUFA to reduce adipose tissue and serum levels of classical endocannabinoids might contribute to a lack of beneficial effects of these lipids on glucose homeostasis in T2DM patients. Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2019
Number of the records: 1