Microfluidic Cultivation and Laser Tweezers Raman Spectroscopy of E-coli under Antibiotic Stress

Pilát, Zdeněk; Bernatová, Silvie; Ježek, Jan; Kirchhoff, J.; Tannert, A.; Neugebauer, U.; Samek, Ota; Zemánek, Pavel

doi:https://dx.doi.org/10.3390/s18051623

Number of the records: 1

Microfluidic Cultivation and Laser Tweezers Raman Spectroscopy of E-coli under Antibiotic Stress

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SYSNO ASEP	0492933
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Microfluidic Cultivation and Laser Tweezers Raman Spectroscopy of E-coli under Antibiotic Stress
Author(s)	Pilát, Zdeněk (UPT-D)_{RID, SAI, ORCID} Bernatová, Silvie (UPT-D)_{RID, SAI} Ježek, Jan (UPT-D)_{RID, ORCID, SAI} Kirchhoff, J. (DE) Tannert, A. (DE) Neugebauer, U. (DE) Samek, Ota (UPT-D)_{RID, ORCID, SAI} Zemánek, Pavel (UPT-D)_{RID, SAI, ORCID}
Number of authors	8
Article number	1623
Source Title	Sensors. - : MDPI Roč. 18, č. 5 (2018), s. 1-14
Number of pages	14 s.
Publication form	Print - P
Language	eng - English
Country	CH - Switzerland
Keywords	Raman micro-spectroscopy ; optical tweezers ; opto-fluidics ; E. coli ; antibiotics
Subject RIV	BH - Optics, Masers, Lasers
OECD category	Microbiology
R&D Projects	GA16-12477S GA ČR - Czech Science Foundation (CSF)
	LO1212 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	ED0017/01/01 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	UPT-D - RVO:68081731
UT WOS	000435580300322
EID SCOPUS	85047257707
DOI	10.3390/s18051623
Annotation	Analyzing the cells in various body fluids can greatly deepen the understanding of the mechanisms governing the cellular physiology. Due to the variability of physiological and metabolic states, it is important to be able to perform such studies on individual cells. Therefore, we developed an optofluidic system in which we precisely manipulated and monitored individual cells of Escherichia coli. We tested optical micromanipulation in a microfluidic chamber chip by transferring individual bacteria into the chambers. We then subjected the cells in the chambers to antibiotic cefotaxime and we observed the changes by using time-lapse microscopy. Separately, we used laser tweezers Raman spectroscopy (LTRS) in a different micro-chamber chip to manipulate and analyze individual cefotaxime-treated E. coli cells. Additionally, we performed conventional Raman micro-spectroscopic measurements of E. coli cells in a micro-chamber. We found observable changes in the cellular morphology (cell elongation) and in Raman spectra, which were consistent with other recently published observations. The principal component analysis (PCA) of Raman data distinguished between the cefotaxime treated cells and control. We tested the capabilities of the optofluidic system and found it to be a reliable and versatile solution for this class of microbiological experiments.
Workplace	Institute of Scientific Instruments
Contact	Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178
Year of Publishing	2019

Number of the records: 1