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3D reconstruction of peripheral nerves from optical projection tomography images: A method for studying fascicular interconnections and intraneural plexuses
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SYSNO ASEP 0491228 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title 3D reconstruction of peripheral nerves from optical projection tomography images: A method for studying fascicular interconnections and intraneural plexuses Author(s) Prats-Galino, A. (ES)
Čapek, Martin (UMG-J)
Reina, M. A. (ES)
Cvetko, E. (SI)
Radochová, Barbora (FGU-C) RID, ORCID
Tubbs, R.S. (GD)
Damjanovska, M. (SI)
Pintaric, T.S. (SI)Number of authors 8 Source Title Clinical Anatomy. - : Wiley - ISSN 0897-3806
Roč. 31, č. 3 (2018), s. 424-431Number of pages 8 s. Language eng - English Country US - United States Keywords 3D reconstructions ; optical projection tomography ; peripheral nerve ; fascicles Subject RIV EB - Genetics ; Molecular Biology OECD category Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology Subject RIV - cooperation Institute of Physiology - Cell Biology R&D Projects GA13-12412S GA ČR - Czech Science Foundation (CSF) LM2015062 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LO1419 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) EF16_013/0001775 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UMG-J - RVO:68378050 ; FGU-C - RVO:67985823 UT WOS 000427113800022 EID SCOPUS 85038120766 DOI 10.1002/ca.23028 Annotation The general microscopic characteristics of nerves are described in several textbooks of histology, but the specific microanatomies of most nerves that can be blocked by anesthesiologists are usually less well known. Our objective was to evaluate the 3D reconstruction of nerve fascicles from optical projection tomography images (OPT) and the ability to undertake an internal navigation exploring the morphology in detail, more specifically the fascicular interconnections. Median and lingual nerve samples were obtained from five euthanized piglets. OPT images of the samples were acquired and 3D reconstruction was performed. The OPT technique revealed the inner structure of the nerves at high resolution, including large and small fascicles, perineurium, interfascicular tissue, and epineurium. The fascicles were loosely packed inside the median nerve and more densely so in the lingual nerve. Analysis of the 3D models demonstrated that the nerve fascicles can show six general spatial patterns. Fascicular interconnections were clearly identified. The 3D reconstruction of nerve fascicles from OPT images opens a new path for research into the microstructure of the inner contents of fascicular nerve groups and their spatial disposition within the nerve including their interconnections. These techniques enable 3D images of partial areas of nerves to be produced and could became an excellent tool for obtaining data concerning the 3D microanatomy of nerves, essential for better interpretation of ultrasound images in clinical practice and thus avoiding possible neurological complications. Clin. Anat. 31:424-431, 2018. (c) 2017 Wiley Periodicals, Inc. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2019
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