Number of the records: 1
Thin-layer chromatography combined with diode laser thermal vaporization inductively coupled plasma mass spectrometry for the determination of selenomethionine and selenocysteine in algae and yeast
- 1.
SYSNO ASEP 0490196 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Thin-layer chromatography combined with diode laser thermal vaporization inductively coupled plasma mass spectrometry for the determination of selenomethionine and selenocysteine in algae and yeast Author(s) Bednařík, A. (CZ)
Kuta, J. (CZ)
Vu, Dai Long (MBU-M) ORCID
Ranglová, Karolína (MBU-M) ORCID
Hrouzek, Pavel (MBU-M) ORCID
Kanický, V. (CZ)
Preisler, J. (CZ)Source Title Journal of Chromatography A. - : Elsevier - ISSN 0021-9673
Roč. 1533, JAN 19 (2018), s. 199-207Number of pages 9 s. Language eng - English Country NL - Netherlands Keywords Diode laser thermal vaporization (DLTV) ; Thin-layer chromatography (TLC) ; Liquid chromatography (LC) Subject RIV EE - Microbiology, Virology OECD category Microbiology R&D Projects LO1416 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support MBU-M - RVO:61388971 UT WOS 000424187500022 EID SCOPUS 85039045431 DOI 10.1016/j.chroma.2017.12.017 Annotation In this work we present a simple and cost-effective approach for the determination of selenium species in algae and yeast biomass, based on a combination of thin-layer chromatography (TLC) with diode laser thermal vaporization inductively coupled plasma mass spectrometry (DLTV ICP MS). Extraction of freeze-dried biomass was performed in 4M methanesulphonic acid and the selenium species were vaporized from cellulose TLC plates employing a continuous-wave infrared diode laser with power up to 4W using a simple laboratory-built apparatus. Selenomethionine and selenocysteine were quantified with limits of detection 3 mu g L-1 in a Se-enriched microalgae Chlorella vulgaris and yeast certified reference material SELM-1. Results delivered by TLC-DLTV ICP MS were consistent with those obtained by a routine coupling of high-performance liquid chromatography (HPLC) to ICP MS. In addition, the TLC approach is capable of analyzing extract containing even undiluted crude hydrolysates that could damage HPLC columns. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2019
Number of the records: 1