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Phytase-mediated enzymatic mineralization of chitosan-enriched hydrogels

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    SYSNO ASEP0489869
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JČlánek ve WOS
    TitlePhytase-mediated enzymatic mineralization of chitosan-enriched hydrogels
    Author(s) Lišková, Jana (FGU-C) RID, ORCID
    Douglas, T.E.L. (BE)
    Wijnants, R. (BE)
    Samal, S. K. (BE)
    Mendez, A. C. (DK)
    Chronakis, I. (DK)
    Bačáková, Lucie (FGU-C) RID, ORCID
    Skirtach, A. G. (BE)
    Source TitleMaterials Letters. - : ELSEVIER SCIENCE BV - ISSN 0167-577X
    Roč. 214, Mar 1 (2018), s. 186-189
    Number of pages4 s.
    Languageeng - English
    CountryNL - Netherlands
    Keywordsbiomaterials ; biomimetic ; composite materials
    Subject RIVEI - Biotechnology ; Bionics
    OECD categoryTechnologies involving identifying the functioning of DNA, proteins and enzymes and how they influence the onset of disease and maintenance of well-being (gene-based diagnostics and therapeutic interventions (pharmacogenomics, gene-based therapeutics)
    R&D ProjectsNV15-33018A GA MZd - Ministry of Health (MZ)
    Institutional supportFGU-C - RVO:67985823
    UT WOS000419724400048
    EID SCOPUS85042236906
    AnnotationHydrogels mineralized with calcium phosphate (CaP) are increasingly popular bone regeneration biomaterials. Mineralization can be achieved by phosphatase enzyme incorporation and incubation in calcium glycerophosphate (CaGP). Gellan gum (GG) hydrogels containing the enzyme phytase and chitosan oligomer were mineralized in CaGP solution and characterized with human osteoblast-like MG63 cells and adipose tissue-derived stem cells (ADSC). Phytase induced CaP formation. Chitosan concentration determined mineralization extent and hydrogel mechanical reinforcement. Phytase-induced mineralization promoted MG63 adhesion and proliferation, especially in the presence of chitosan, and was non-toxic to MG63 cells (with and without chitosan). ADSC adhesion and proliferation were poor without mineralization. Chitosan did not affect ADSC osteogenic differentiation.
    WorkplaceInstitute of Physiology
    ContactLucie Trajhanová,, Tel.: 241 062 400
    Year of Publishing2019
Number of the records: 1