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Phytase-mediated enzymatic mineralization of chitosan-enriched hydrogels
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SYSNO ASEP 0489869 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Phytase-mediated enzymatic mineralization of chitosan-enriched hydrogels Author(s) Lišková, Jana (FGU-C) RID, ORCID
Douglas, T.E.L. (BE)
Wijnants, R. (BE)
Samal, S. K. (BE)
Mendez, A. C. (DK)
Chronakis, I. (DK)
Bačáková, Lucie (FGU-C) RID, ORCID
Skirtach, A. G. (BE)Source Title Materials Letters. - : ELSEVIER SCIENCE BV - ISSN 0167-577X
Roč. 214, Mar 1 (2018), s. 186-189Number of pages 4 s. Language eng - English Country NL - Netherlands Keywords biomaterials ; biomimetic ; composite materials Subject RIV EI - Biotechnology ; Bionics OECD category Technologies involving identifying the functioning of DNA, proteins and enzymes and how they influence the onset of disease and maintenance of well-being (gene-based diagnostics and therapeutic interventions (pharmacogenomics, gene-based therapeutics) R&D Projects NV15-33018A GA MZd - Ministry of Health (MZ) Institutional support FGU-C - RVO:67985823 UT WOS 000419724400048 EID SCOPUS 85042236906 DOI https://doi.org/10.1016/j.matlet.2017.12.004 Annotation Hydrogels mineralized with calcium phosphate (CaP) are increasingly popular bone regeneration biomaterials. Mineralization can be achieved by phosphatase enzyme incorporation and incubation in calcium glycerophosphate (CaGP). Gellan gum (GG) hydrogels containing the enzyme phytase and chitosan oligomer were mineralized in CaGP solution and characterized with human osteoblast-like MG63 cells and adipose tissue-derived stem cells (ADSC). Phytase induced CaP formation. Chitosan concentration determined mineralization extent and hydrogel mechanical reinforcement. Phytase-induced mineralization promoted MG63 adhesion and proliferation, especially in the presence of chitosan, and was non-toxic to MG63 cells (with and without chitosan). ADSC adhesion and proliferation were poor without mineralization. Chitosan did not affect ADSC osteogenic differentiation. Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2019
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