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Phytase-mediated enzymatic mineralization of chitosan-enriched hydrogels

SYSNO ASEP	0489869
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Phytase-mediated enzymatic mineralization of chitosan-enriched hydrogels
Author(s)	Lišková, Jana (FGU-C)_{RID, ORCID} Douglas, T.E.L. (BE) Wijnants, R. (BE) Samal, S. K. (BE) Mendez, A. C. (DK) Chronakis, I. (DK) Bačáková, Lucie (FGU-C)_{RID, ORCID} Skirtach, A. G. (BE)
Source Title	Materials Letters. - : ELSEVIER SCIENCE BV - ISSN 0167-577X Roč. 214, Mar 1 (2018), s. 186-189
Number of pages	4 s.
Language	eng - English
Country	NL - Netherlands
Keywords	biomaterials ; biomimetic ; composite materials
Subject RIV	EI - Biotechnology ; Bionics
OECD category	Technologies involving identifying the functioning of DNA, proteins and enzymes and how they influence the onset of disease and maintenance of well-being (gene-based diagnostics and therapeutic interventions (pharmacogenomics, gene-based therapeutics)
R&D Projects	NV15-33018A GA MZd - Ministry of Health (MZ)
Institutional support	FGU-C - RVO:67985823
UT WOS	000419724400048
EID SCOPUS	85042236906
DOI	10.1016/j.matlet.2017.12.004
Annotation	Hydrogels mineralized with calcium phosphate (CaP) are increasingly popular bone regeneration biomaterials. Mineralization can be achieved by phosphatase enzyme incorporation and incubation in calcium glycerophosphate (CaGP). Gellan gum (GG) hydrogels containing the enzyme phytase and chitosan oligomer were mineralized in CaGP solution and characterized with human osteoblast-like MG63 cells and adipose tissue-derived stem cells (ADSC). Phytase induced CaP formation. Chitosan concentration determined mineralization extent and hydrogel mechanical reinforcement. Phytase-induced mineralization promoted MG63 adhesion and proliferation, especially in the presence of chitosan, and was non-toxic to MG63 cells (with and without chitosan). ADSC adhesion and proliferation were poor without mineralization. Chitosan did not affect ADSC osteogenic differentiation.
Workplace	Institute of Physiology
Contact	Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
Year of Publishing	2019

Number of the records: 1