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RNase III-Binding-mRNAs Revealed Novel Complementary Transcripts in Streptomyces
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SYSNO ASEP 0488899 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title RNase III-Binding-mRNAs Revealed Novel Complementary Transcripts in Streptomyces Author(s) Šetinová, D. (CZ)
Šmídová, K. (CZ)
Pohl, P. (CZ)
Music, I. (CZ)
Bobek, Jan (MBU-M)Article number 2693 Source Title Frontiers in Microbiology. - : Frontiers Research Foundation - ISSN 1664-302X
Roč. 8, JAN 15 2018 (2018)Number of pages 12 s. Language eng - English Country CH - Switzerland Keywords cis-antisense RNA ; RNase III ; Streptomyces Subject RIV EE - Microbiology, Virology OECD category Microbiology R&D Projects LM2015055 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support MBU-M - RVO:61388971 UT WOS 000419881100006 EID SCOPUS 85040812843 DOI 10.3389/fmicb.2017.02693 Annotation cis-Antisense RNAs (asRNAs) provide very simple and effective gene expression control due to the perfect complementarity between regulated and regulatory transcripts. In Streptomyces, the antibiotic-producing clade, the antisense control system is not yet understood, although it might direct the organism's complex development. Initial studies in Streptomyces have found a number of asRNAs. Apart from this, hundreds of mRNAs have been shown to bind RNase III, the double strand-specific endoribonuclease. In this study, we tested 17 mRNAs that have been previously co-precipitated with RNase III for antisense expression. Our RACE mapping showed that all of these mRNAs possess cognate asRNA. Additional tests for antisense expression uncovered as-adpA, as-rnc, as3983, as-sigB, as-sigH, and as-sigR RNAs. Northern blots detected the expression profiles of 18 novel transcripts. Noteworthy, we also found that only a minority of asRNAs respond to the absence of RNase III enzyme by increasing their cellular levels. Our findings suggest that antisense expression is widespread in Streptomyces, including genes of such important developmental regulators, as AdpA, RNase III, and sigma factors. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2019
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