Number of the records: 1

Exocyst subunit EXO70H4 has a specific role in callose synthase secretion and silica accumulation

SYSNO ASEP	0488738
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Exocyst subunit EXO70H4 has a specific role in callose synthase secretion and silica accumulation
Author(s)	Kulich, I. (CZ) Vojtíková, Z. (CZ) Sabol, P. (CZ) Ortmannová, Jitka (UEB-Q)_ORCID Neděla, Vilém (UPT-D)_{RID, ORCID, SAI} Tihlaříková, Eva (UPT-D)_{RID, ORCID, SAI} Žárský, Viktor (UEB-Q)_{RID, ORCID}
Number of authors	7
Source Title	Plant Physiology. - : Oxford University Press - ISSN 0032-0889 Roč. 176, č. 3 (2018), s. 2040-2051
Number of pages	12 s.
Language	eng - English
Country	US - United States
Keywords	COMPLETE PENETRATION RESISTANCE ; SCANNING-ELECTRON-MICROSCOPE ; POWDERY MILDEW
Subject RIV	EB - Genetics ; Molecular Biology
OECD category	Cell biology
Subject RIV - cooperation	Institute of Scientific Instruments - Electronics ; Optoelectronics, Electrical Engineering
Institutional support	UEB-Q - RVO:61389030 ; UPT-D - RVO:68081731
UT WOS	000426848300018
EID SCOPUS	85044003089
DOI	10.1104/pp.17.01693
Annotation	Biogenesis of the plant secondary cell wall involves many important aspects, such as phenolic compound deposition and often silica encrustation. Previously, we demonstrated the importance of the exocyst subunit EXO70H4 for biogenesis of the trichome secondary cell wall, namely for deposition of the autofluorescent and callose-rich cell wall layer. Here, we reveal that EXO70H4-driven cell wall biogenesis is cons titutively active in the mature trichome, but also can be activated elsewhere upon pathogen attack, giving this study a broader significance with an overlap into phytopathology. To address the specificity of EXO70H4 among the EXO70 family, we complemented the exo70H4-1 mutant by 18 different Arabidopsis (Arabidopsis thaliana) EXO70 paralogs subcloned under the EXO70H4 promoter. Only EXO70H4 had the capacity to rescue the exo70H4-1 trichome phenotype. Callose deposition phenotype of exo70H4-1 mutant is caused by impaired secretion of PMR4, a callose synthase responsible for the synthesis of callose in the trichome. PMR4 colocalizes with EXO70H4 on plasma membrane microdomains that do not develop in the exo70H4-1 mutant. Using energy-dispersive x-ray microanalysis, we show that both EXO70H4- and PMR4-dependent callose deposition in the trichome are essential for cell wall silicification.
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2019

Number of the records: 1